PUBLICATION
Loss of wwox expression in zebrafish embryos causes edema and alters Ca(2+) dynamics
- Authors
- Tsuruwaka, Y., Konishi, M., Shimada, E.
- ID
- ZDB-PUB-150205-9
- Date
- 2015
- Source
- PeerJ 3: e727 (Journal)
- Registered Authors
- Keywords
- Intracellular calcium, WWOX, Yellow cameleon, Zebrafish
- MeSH Terms
- none
- PubMed
- 25649963 Full text @ Peer J.
Citation
Tsuruwaka, Y., Konishi, M., Shimada, E. (2015) Loss of wwox expression in zebrafish embryos causes edema and alters Ca(2+) dynamics. PeerJ. 3:e727.
Abstract
We investigated the role of the WW domain-containing oxidoreductase (wwox) gene in the embryonic development of zebrafish, with particular emphasis on intracellular Ca(2+) dynamics because Ca(2+) is an important intracellular messenger. Comparisons between zebrafish wwox and human WWOX sequences identified highly conserved domain structures. wwox was expressed in developing heart tissues in the zebrafish embryo. Moreover, wwox knockdown induced pericardial edema with similarities to conditions observed in human breast cancer. The wwox knockdown embryos with the edema died within a week. High Ca(2+) levels were observed at the boundary between the edema and yolk in wwox knockdown embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping