PUBLICATION
A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance
- Authors
- Gabor, K., Charette, J., Pietraszewski, M., Wingfield, D., Shim, J., Millard, P., Kim, C.
- ID
- ZDB-PUB-150131-1
- Date
- 2015
- Source
- Developmental and comparative immunology 51(2): 298-304 (Journal)
- Registered Authors
- Kim, Carol H.
- Keywords
- Danio rerio, Type I IFN, Zebrafish, antiviral, mda5, rhabdovirus
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- DEAD-box RNA Helicases/genetics
- DEAD-box RNA Helicases/immunology
- DEAD-box RNA Helicases/metabolism*
- Immunity, Active/genetics
- Interferon Type I/metabolism
- Mutation/genetics
- Receptors, Pattern Recognition/genetics
- Receptors, Pattern Recognition/immunology
- Receptors, Pattern Recognition/metabolism*
- Rhabdoviridae/immunology*
- Rhabdoviridae Infections/immunology*
- Transgenes/genetics
- Viral Load/genetics
- Zebrafish/immunology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology
- Zebrafish Proteins/metabolism*
- PubMed
- 25634485 Full text @ Dev. Comp. Immunol.
Citation
Gabor, K., Charette, J., Pietraszewski, M., Wingfield, D., Shim, J., Millard, P., Kim, C. (2015) A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance. Developmental and comparative immunology. 51(2):298-304.
Abstract
Melanoma Differentiation-Associated protein 5 (MDA5) is a member of the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family, which are cytosolic pattern recognition receptors that detect viral nucleic acids. Here we show an Mda5-dependent response to rhabdovirus infection in vivo using a dominant-negative mda5 transgenic zebrafish. Dominant-negative mda5 zebrafish embryos displayed an impaired antiviral immune response compared to wild-type counterparts that can be rescued by recombinant full-length Mda5. To our knowledge, we have generated the first dominant-negative mda5 transgenic zebrafish and demonstrated a critical role for Mda5 in the antiviral response to rhabdovirus.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping