|ZFIN ID: ZDB-PUB-150128-1|
High-efficiency In Vitro and In Vivo Detection of Zn2+ by Dye-assembled Upconversion Nanoparticles
Peng, J., Xu, W., Teoh, C.L., Han, S., Kim, B., Samanta, A., Er, J.C., Wang, L., Yuan, L., Liu, X., Chang, Y.T.
|Source:||Journal of the American Chemical Society 137(6): 2336-42 (Journal)|
|Registered Authors:||Wang, Lu|
|PubMed:||25626163 Full text @ J. Am. Chem. Soc.|
Peng, J., Xu, W., Teoh, C.L., Han, S., Kim, B., Samanta, A., Er, J.C., Wang, L., Yuan, L., Liu, X., Chang, Y.T. (2015) High-efficiency In Vitro and In Vivo Detection of Zn2+ by Dye-assembled Upconversion Nanoparticles. Journal of the American Chemical Society. 137(6):2336-42.
ABSTRACTDevelopment of highly sensitive and selective sensing systems of divalent zinc ion (Zn2+) in organisms has been a growing interest in the past decades owing to its pivotal role in cellular metabolism, apoptosis and neurotransmission. Herein, we report the rational design and synthesis of a Zn2+ fluorescent-based probe by assembling lanthanide-doped upconversion nanoparticles (UCNPs) with chromophores. Specifically, upconversion luminescence (UCL) can be effectively quenched by the chromophores on the surface of nanoparticles via a fluorescence resonant energy transfer (FRET) process and subsequently recovered upon the addition of Zn2+, thus allowing for quantitative monitoring of Zn2+. Importantly, the sensing system enables detection of Zn2+ in real biological samples. We demonstrate that this chromophore-UCNP nanosystem is capable of implementing an efficient in vitro and in vivo detection of Zn2+ in mouse brain slice with Alzheimer's disease and zebrafish, respectively.
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