ZFIN ID: ZDB-PUB-150128-1
High-efficiency In Vitro and In Vivo Detection of Zn2+ by Dye-assembled Upconversion Nanoparticles
Peng, J., Xu, W., Teoh, C.L., Han, S., Kim, B., Samanta, A., Er, J.C., Wang, L., Yuan, L., Liu, X., Chang, Y.T.
Date: 2015
Source: Journal of the American Chemical Society   137(6): 2336-42 (Journal)
Registered Authors: Wang, Lu
Keywords: none
MeSH Terms:
  • Cell Line, Tumor
  • Coloring Agents/chemistry*
  • Humans
  • In Vitro Techniques
  • Nanoparticles*
  • Zinc/analysis*
PubMed: 25626163 Full text @ J. Am. Chem. Soc.
Development of highly sensitive and selective sensing systems of divalent zinc ion (Zn2+) in organisms has been a growing interest in the past decades owing to its pivotal role in cellular metabolism, apoptosis and neurotransmission. Herein, we report the rational design and synthesis of a Zn2+ fluorescent-based probe by assembling lanthanide-doped upconversion nanoparticles (UCNPs) with chromophores. Specifically, upconversion luminescence (UCL) can be effectively quenched by the chromophores on the surface of nanoparticles via a fluorescence resonant energy transfer (FRET) process and subsequently recovered upon the addition of Zn2+, thus allowing for quantitative monitoring of Zn2+. Importantly, the sensing system enables detection of Zn2+ in real biological samples. We demonstrate that this chromophore-UCNP nanosystem is capable of implementing an efficient in vitro and in vivo detection of Zn2+ in mouse brain slice with Alzheimer's disease and zebrafish, respectively.