|ZFIN ID: ZDB-PUB-150127-6|
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Zebrafish Rnf111 is encoded by multiple transcripts and is required for epiboly progression and prechordal plate development
Bessarab, D.A., Mathavan, S., Jones, C.M., Ray Dunn, N.
|Source:||Differentiation; research in biological diversity 89(1-2): 22-30 (Journal)|
|Registered Authors:||Bessarab, Dmitri, Mathavan, S.|
|Keywords:||Alternative splicing, Epiboly, Evolutionary conservation, Prechordal plate, Zebrafish, rnf111|
|PubMed:||25619648 Full text @ Differentiation|
Bessarab, D.A., Mathavan, S., Jones, C.M., Ray Dunn, N. (2015) Zebrafish Rnf111 is encoded by multiple transcripts and is required for epiboly progression and prechordal plate development. Differentiation; research in biological diversity. 89(1-2):22-30.
ABSTRACTArkadia (also known as RING finger 111) encodes a nuclear E3 ubiquitin ligase that targets intracellular effectors and modulators of TGFβ/Nodal-related signaling for polyubiquitination and proteasome-dependent degradation. In the mouse, loss of Arkadia results in early embryonic lethality, with defects attributed to compromised Nodal signaling. Here, we report the isolation of zebrafish arkadia/rnf111, which is represented by 5 transcript variants. arkadia/rnf111 is broadly expressed during the blastula and gastrula stages, with eventual enrichment in the anterior mesendoderm, including the prechordal plate. Morpholino knockdown experiments reveal an unexpected role for Arkadia/Rnf111 in both early blastula organization and epiboly progression. Using a splice junction morpholino, we present additional evidence that arkadia/rnf111 transcript variants containing a 3' alternative exon are specifically required for epiboly progression in the late gastrula. This result suggests that arkadia/rnf111 transcript variants encode functionally relevant protein isoforms that provide additional intracellular flexibility and regulation to the Nodal signaling pathway.