PUBLICATION

Magnetic-activated cell sorting (MACS) can be used as a large-scale method for establishing zebrafish neuronal cell cultures

Authors
Welzel, G., Seitz, D., Schuster, S.
ID
ZDB-PUB-150123-9
Date
2015
Source
Scientific Reports   5: 7959 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Animals
  • Cell Aggregation/drug effects
  • Cell Differentiation/drug effects
  • Cell Separation
  • Cells, Cultured
  • Embryo, Nonmammalian/cytology*
  • Flow Cytometry/methods*
  • Nerve Net/drug effects
  • Nerve Net/metabolism
  • Neural Stem Cells/cytology
  • Neural Stem Cells/drug effects
  • Neural Stem Cells/metabolism
  • Neurons/cytology*
  • Neurons/drug effects
  • Neurons/metabolism
  • Tretinoin/pharmacology
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism
PubMed
25609542 Full text @ Sci. Rep.
Abstract
Neuronal cell cultures offer a crucial tool to mechanistically analyse regeneration in the nervous system. Despite the increasing importance of zebrafish (Danio rerio) as an in vivo model in neurobiological and biomedical research, in vitro approaches to the nervous system are lagging far behind and no method is currently available for establishing enriched neuronal cell cultures. Here we show that magnetic-activated cell sorting (MACS) can be used for the large-scale generation of neuronal-restricted progenitor (NRP) cultures from embryonic zebrafish. Our findings provide a simple and semi-automated method that is likely to boost the use of neuronal cell cultures as a tool for the mechanistic dissection of key processes in neuronal regeneration and development.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping