ZFIN ID: ZDB-PUB-150122-10
TGFβ1a regulates zebrafish posterior lateral line formation via Smad5 mediated pathway
Xing, C., Gong, B., Xue, Y., Han, Y., Wang, Y., Meng, A., Jia, S.
Date: 2015
Source: Journal of molecular cell biology   7(1): 48-61 (Journal)
Registered Authors: Gong, Bo, Han, Yanchao, Jia, Shunji, Meng, Anming, Xing, Cencan
Keywords: Smad5, Tgfβ1a, lateral line, neuromast, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Apoptosis/genetics
  • Body Patterning/genetics*
  • Cadherins/genetics
  • Cadherins/metabolism
  • Cell Differentiation/genetics
  • Chemokines/genetics
  • Chemokines/metabolism
  • Gene Expression Regulation, Developmental*
  • Gene Knockdown Techniques
  • Mutation
  • Phenotype
  • Signal Transduction*
  • Smad5 Protein/metabolism*
  • Transforming Growth Factor beta1/genetics*
  • Transforming Growth Factor beta1/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism*
PubMed: 25603803 Full text @ J. Mol. Cell Biol.
The zebrafish sensory posterior lateral line (pLL) has become an attractive model for studying collective cell migration and cell morphogenesis. Recent studies have indicated that chemokine, Wnt/β-catenin, Fgf, and Delta-Notch signaling pathways participate in regulating pLL development. However, it remains unclear whether TGFβ signaling pathway is involved in pLL development. Here we report a critical role of Tgfβ1 in regulating morphogenesis of the pLL primordium (pLLP). The tgfβ1a gene is abundantly expressed in the lateral line primordium. Knockdown or knockout of tgfβ1a leads to a reduction of neuromast number, an increase of inter-neuromast distance, and a reduced number of hair cells. The aberrant morphogenesis in embryos depleted of tgfβ1a correlates with the reduced expression of atoh1a, delta, and n-cadherin/cdh2, which are known important regulators of the pLLP morphogenesis. Like tgfβ1a depletion, knockdown of smad5 that expresses in the pLLP, affects pLLP development whereas overexpression of a constitutive active Smad5 isoform rescues the defects in embryos depleted of tgfβ1a, indicating that Smad5 mediates tgfβ1a function in pLLP development. Therefore, Tgfβ/Smad5 signaling plays an important role in the zebrafish lateral line formation.