PUBLICATION

Characterization of two thymosins as immune-related genes in common carp (cyprinus carpio l.)

Authors
Xiao, Z., Shen, J., Feng, H., Liu, H., Wang, Y., Huang, R., Guo, Q.
ID
ZDB-PUB-150118-2
Date
2015
Source
Developmental and comparative immunology   50(1): 29-37 (Journal)
Registered Authors
Keywords
beta thymosin (Tβ), common carp, prothymosin alpha (ProTα), spring viraemia of carp virus (SVCV)
MeSH Terms
  • Animals
  • Carps/genetics*
  • Carps/immunology
  • Fish Proteins/genetics*
  • Fish Proteins/immunology
  • Protein Precursors/genetics*
  • Protein Precursors/immunology
  • T-Lymphocytes/immunology
  • Thymosin/analogs & derivatives*
  • Thymosin/genetics
  • Thymosin/immunology
  • Ubiquitins/genetics*
  • Ubiquitins/immunology
  • Zebrafish/genetics
PubMed
25596145 Full text @ Dev. Comp. Immunol.
Abstract
Prothymosin alpha (ProTα) and thymosin beta (Tβ) belong to thymosin family, which consists of a series of highly conserved peptides involved in stimulating immune responses. ProTα b and Tβ are still poorly studied in teleost. Here, the full-length cDNAs of ProTα b and Tβ-like (Tβ-l) were cloned and identified in common carp (Cyprinus carpio L.). The expressions of carp ProTα b and Tβ-l exhibited rise-fall pattern and then trended to be stable during early development. After spring viraemia of carp virus (SVCV) infection, the carp ProTα b and Tβ-l transcripts were significantly up-regulated in some immune-related organs. When transiently over-expressed carp ProTα b and Tβ-l in zebrafish, these two proteins up-regulated the expressions of T lymphocytes-related genes (Rag 1, TCR-γ, CD4 and CD8α). These results suggest that carp ProTα b and Tβ may ultimately enhance the immune response during viral infection and modulate the development of T lymphocytes in teleost.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping