PUBLICATION
Protective role of quercetin against cisplatin-induced hair cell damage in zebrafish embryos
- Authors
- Lee, S., Oh, K., Chung, A., Park, H., Lee, S., Kwon, S., Choi, J.
- ID
- ZDB-PUB-150117-6
- Date
- 2015
- Source
- Human & Experimental Toxicology 34(11): 1043-52 (Journal)
- Registered Authors
- Keywords
- Quercetin, cisplatin, hair cell, ototoxicity, zebrafish
- MeSH Terms
-
- Animals
- Antineoplastic Agents/adverse effects*
- Apoptosis/drug effects
- Cisplatin/adverse effects*
- Embryo, Nonmammalian
- Hair Cells, Auditory/drug effects*
- Hair Cells, Auditory/ultrastructure
- Microscopy, Electron, Scanning
- Mitochondria/drug effects
- Protective Agents/pharmacology*
- Quercetin/pharmacology*
- Zebrafish
- PubMed
- 25591968 Full text @ Hum. Exp. Toxicol.
Citation
Lee, S., Oh, K., Chung, A., Park, H., Lee, S., Kwon, S., Choi, J. (2015) Protective role of quercetin against cisplatin-induced hair cell damage in zebrafish embryos. Human & Experimental Toxicology. 34(11):1043-52.
Abstract
Background and objectives The aim of this study was to evaluate the protective effects of quercetin on cisplatin-induced hair cell damage in transgenic zebrafish embryos.
Materials and methods Five days postfertilization zebrafish embryos were exposed to 1 mM cisplatin and quercetin at 10, 50, 100, or 200 μM for 4 h. Hair cells within neuromasts of the supraorbital, otic, and occipital lateral lines were analyzed by fluorescent microscopy (n = 10). Survival of hair cells was calculated as the average number of hair cells in the control group that were not exposed to cisplatin. Ultrastructural changes were evaluated using scanning electron microscopy.
Results Hair cell damage in neuromasts was decreased by co-treatment of quercetin and cisplatin (quercetin 100 μM: 8.6 ± 1.1 cells; 1 mM cisplatin only: 5.0 ± 0.5 cells; n = 10, p < 0.05); apoptosis of hair cells examined by special stain was also decreased by quercetin. The ultrastructure of hair cells within neuromasts was preserved in zebrafish by the combination of quercetin (100 μM) and cisplatin (1 mM).
Conclusion In conclusion, quercetin showed protective effects against cisplatin-induced toxicity in a zebrafish model. The results of this study suggest the possibility of a protective role of quercetin against cisplatin-induced apoptotic cell death in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping