PUBLICATION
Igfs mediate the action of luteinizing hormone on oocyte maturation in zebrafish
- Authors
- Li, J., Chu, L., Sun, X., Liu, Y., Cheng, C.H.
- ID
- ZDB-PUB-150115-12
- Date
- 2015
- Source
- Molecular endocrinology (Baltimore, Md.) 29(3): 373-83 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation*/drug effects
- Cell Differentiation*/genetics
- Cells, Cultured
- Chorionic Gonadotropin/pharmacology
- Female
- Gene Expression Profiling
- Gene Expression Regulation, Developmental/drug effects
- Gene Knockout Techniques
- Humans
- Immune Sera
- Insulin-Like Growth Factor I
- Luteinizing Hormone, beta Subunit/metabolism*
- Models, Biological
- Mutation/genetics
- Oocytes/cytology*
- Oocytes/drug effects
- Oocytes/metabolism
- Organogenesis/drug effects
- Organogenesis/genetics
- Ovarian Follicle/drug effects
- Ovarian Follicle/growth & development
- Ovarian Follicle/metabolism
- Phosphorylation/drug effects
- Receptor, IGF Type 1/metabolism
- Recombinant Proteins/pharmacology
- Somatomedins/genetics
- Somatomedins/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 25584412 Full text @ Mol. Endocrinol.
Citation
Li, J., Chu, L., Sun, X., Liu, Y., Cheng, C.H. (2015) Igfs mediate the action of luteinizing hormone on oocyte maturation in zebrafish. Molecular endocrinology (Baltimore, Md.). 29(3):373-83.
Abstract
Luteinizing hormone (Lh) signaling is required for oocyte maturation in fish and other vertebrates. However, the downstream factors mediating Lh signaling are largely unexplored in fish. In this study we investigated whether insulin-like growth factors (Igfs) could mediate Lh action on oocyte maturation in zebrafish. Our results show that all igfs including igf1, igf2a, igf2b and igf3 are dynamically expressed during folliculogenesis, with the expression of igf3 reaching its maximal level in full grown stage follicles. The expression of igfs is regulated by Lh through a cAMP pathway in intact follicles as well as in primary cultured follicular cells, with igf3 expression being the most sensitive to hCG treatment. Moreover, recombinant zebrafish Igf2a, Igf2b and Igf3 proteins significantly enhanced oocyte maturation via Igf type 1 receptors (Igf1rs), with Igf3 exhibiting the most potent stimulatory action on oocyte maturation. Furthermore, we have demonstrated that Igf3 or hCG treatment could stimulate Igf1rs phosphorylation and hCG-induced oocyte maturation could be attenuated by Igf1r inhibitors as well as by an anti-Igf3 antiserum in vitro and in vivo, indicating that the Igf system especially Igf3 plays a crucial role in mediating Lh action on oocyte maturation. In addition, igf3 expression is significantly attenuated in luteinizing hormone β-subunit (lhb) mutant zebrafish and treatment with recombinant Igf3 could partially rescue the oocyte maturation defects of the lhb mutants in vitro and in vivo. Collectively, our results clearly demonstrated that Igfs, particularly the gonad-specific Igf3, act as important mediators of Lh action on oocyte maturation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping