PUBLICATION

Fgf-signaling-dependent sox9a and atoh1a regulate otic neural development in zebrafish

Authors
Wang, J., Wu, Y., Zhao, F., Wu, Y., Dong, W., Zhao, J., Zhu, Z., Liu, D.
ID
ZDB-PUB-150109-4
Date
2015
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   35: 234-44 (Journal)
Registered Authors
Liu, Dong, Zhao, Feng, Zhao, Jue
Keywords
Fgf signaling, Fgfr-Erk1/2, Fgfr-PI3K/Akt, hair cells, statoacoustic ganglia
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Ear, Inner/embryology*
  • Ear, Inner/physiology*
  • Embryonic Development/physiology
  • Fibroblast Growth Factors/physiology*
  • Ganglia, Parasympathetic/embryology*
  • Ganglia, Parasympathetic/physiology*
  • Receptors, Fibroblast Growth Factor/physiology
  • SOX9 Transcription Factor/physiology*
  • Signal Transduction/physiology
  • Transcription Factors/physiology*
  • Zebrafish
  • Zebrafish Proteins/physiology*
PubMed
25568117 Full text @ J. Neurosci.
Abstract
Fibroblast growth factors (Fgfs) play important roles in developmental processes of the inner ear, including the ontogeny of the statoacoustic ganglia (SAG) and hair cells. However, the detailed genetic mechanism(s) underlying Fgf/Fgfr-dependent otic neural development remains elusive. Using conditional genetic approaches and inhibitory small molecules, we have revealed that Fgfr-PI3K/Akt signaling is mainly responsible for zebrafish SAG development and have determined that Sox9a and Atoh1a act downstream of Fgfr-Akt signaling to specify and/or maintain the otic neuron fate during the early segmentation stage. Sox9a and Atoh1a coregulate numerous downstream factors identified through our ChIP-seq analyses, including Tlx2 and Eya2. Fgfr-Erk1/2 signaling contributes to ultricular hair cell development during a critical period between 9 and 15 hours postfertilization. Our work reveals that a genetic network of the previously known sensory determinant Atoh1 and the neural crest determinant Sox9 plays critical roles in SAG development. These newly uncovered roles for Atoh1and Sox9 in zebrafish otic development may be relevant to study in other species.
Genes / Markers
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Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes