ZFIN ID: ZDB-PUB-150106-17
Filopodia-based Wnt transport during vertebrate tissue patterning
Stanganello, E., Hagemann, A.I., Mattes, B., Sinner, C., Meyen, D., Weber, S., Schug, A., Raz, E., Scholpp, S.
Date: 2015
Source: Nature communications   6: 5846 (Journal)
Registered Authors: Hagemann, Anja, Mattes, Banjamin, Meyen, Dana, Raz, Erez, Scholpp, Steffen, Stanganello, Eliana, Weber, Sabrina
Keywords: none
MeSH Terms:
  • Animals
  • Body Patterning/physiology*
  • Computer Simulation
  • Cytoskeletal Proteins/metabolism*
  • Fibroblasts/metabolism
  • HEK293 Cells
  • Humans
  • In Situ Hybridization
  • Mice
  • Microscopy, Confocal
  • Neural Plate/embryology*
  • Oligonucleotides, Antisense/genetics
  • Plasmids/genetics
  • Protein Transport/physiology
  • Pseudopodia/physiology*
  • Real-Time Polymerase Chain Reaction
  • Signal Transduction/physiology*
  • Wnt Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism*
  • cdc42 GTP-Binding Protein/metabolism
PubMed: 25556612 Full text @ Nat. Commun.
Paracrine Wnt/β-catenin signalling is important during developmental processes, tissue regeneration and stem cell regulation. Wnt proteins are morphogens, which form concentration gradients across responsive tissues. Little is known about the transport mechanism for these lipid-modified signalling proteins in vertebrates. Here we show that Wnt8a is transported on actin-based filopodia to contact responding cells and activate signalling during neural plate formation in zebrafish. Cdc42/N-Wasp regulates the formation of these Wnt-positive filopodia. Enhanced formation of filopodia increases the effective signalling range of Wnt by facilitating spreading. Consistently, reduction in filopodia leads to a restricted distribution of the ligand and a limited signalling range. Using a simulation, we provide evidence that such a short-range transport system for Wnt has a long-range signalling function. Indeed, we show that a filopodia-based transport system for Wnt8a controls anteroposterior patterning of the neural plate during vertebrate gastrulation.