PUBLICATION
Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis
- Authors
- Yang, X., Yue, H., Ye, H., Li, C., Wei, Q.
- ID
- ZDB-PUB-150101-8
- Date
- 2015
- Source
- Gene 558(1): 118-25 (Journal)
- Registered Authors
- Keywords
- Chinese sturgeon, dead end, fluorescent in situ hybridization, primordial germ cell, qRT-PCR
- MeSH Terms
-
- Sequence Alignment
- Gene Expression
- Molecular Sequence Data
- Fish Proteins/analysis
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Germ Cells/metabolism
- In Situ Hybridization, Fluorescence
- Biomarkers
- Organ Specificity
- Amino Acid Sequence
- Phylogeny
- RNA-Binding Proteins/analysis
- RNA-Binding Proteins/genetics*
- RNA-Binding Proteins/metabolism
- Base Sequence
- Perciformes/embryology
- Perciformes/genetics*
- Perciformes/metabolism
- Cloning, Molecular
- Animals
- Zebrafish
- PubMed
- 25550043 Full text @ Gene
Citation
Yang, X., Yue, H., Ye, H., Li, C., Wei, Q. (2015) Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis. Gene. 558(1):118-25.
Abstract
Dead end (dnd) encodes an RNA-binding protein that is essential for primordial germ cell (PGC) migration and gametogenesis in vertebrates. In this study, a Chinese sturgeon Acipenser sinensis dead end homologue, designated Asdnd, was identified and characterized. The full-length cDNA of Asdnd was 1630 base pairs (bp) and encoded a peptide of 396 amino acid residues. Multiple sequence alignment showed that AsDnd shared six conserved regions of Dnd orthologs, including the RNA recognition motif. Phylogenetic analysis revealed that AsDnd was grouped with teleosts. By quantitative real-time PCR analysis, the Asdnd transcripts were found to originate from the maternal parent and be specifically expressed in gonads of immature Chinese sturgeons of both sexes. Fluorescent in situ hybridization indicated that Asdnd transcripts were restricted to germ cells. In the testis, Asdnd was abundant in spermatogonia and tended to gradually diminish in late spermatogenic stages, while strong signals were found in primary oocytes, as opposed to oogonia, in the ovary. Zebrafish PGCs were clearly visualized at 24hours post-fertilization by co-injecting RFP-Asdnd 3' UTR and GFP-nos3 3' UTR mRNA, indicating that dnd 3' UTR has a conserved function among teleosts. Therefore, dnd could serve as a germ cell marker in Chinese sturgeon.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping