PUBLICATION
Endothelial cell-initiated extravasation of cancer cells visualized in zebrafish
- Authors
- Kanada, M., Zhang, J., Yan, L., Sakurai, T., Terakawa, S.
- ID
- ZDB-PUB-150101-3
- Date
- 2014
- Source
- PeerJ 2: e688 (Journal)
- Registered Authors
- Keywords
- Cancer cell, Endothelial cell, Extravasation, Fluorescence, In vivo imaging, Metastasis, Sunitinib, Tumor cell, VEGF, Zebrafish
- MeSH Terms
- none
- PubMed
- 25551022 Full text @ Peer J.
Citation
Kanada, M., Zhang, J., Yan, L., Sakurai, T., Terakawa, S. (2014) Endothelial cell-initiated extravasation of cancer cells visualized in zebrafish. PeerJ. 2:e688.
Abstract
The extravasation of cancer cells, a key step for distant metastasis, is thought to be initiated by disruption of the endothelial barrier by malignant cancer cells. An endothelial covering-type extravasation of cancer cells in addition to conventional cancer cell invasion-type extravasation was dynamically visualized in a zebrafish hematogenous metastasis model. The inhibition of VEGF-signaling impaired the invasion-type extravasation via inhibition of cancer cell polarization and motility. Paradoxically, the anti-angiogenic treatment showed the promotion, rather than the inhibition, of the endothelial covering-type extravasation of cancer cells, with structural changes in the endothelial walls. These findings may be a set of clues to the full understanding of the metastatic process as well as the metastatic acceleration by anti-angiogenic reagents observed in preclinical studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping