PUBLICATION
A zebrafish (Danio rerio) bloodthirsty member 20 with E3 ubiquitin ligase activity involved in immune response against bacterial infection
- Authors
- Zhang, X., Zhao, H., Chen, Y., Luo, H., Yang, P., Yao, B.
- ID
- ZDB-PUB-141230-14
- Date
- 2015
- Source
- Biochemical and Biophysical Research Communications 457(1): 83-9 (Journal)
- Registered Authors
- Keywords
- Bloodthirsty member 20 (btr20), E3 ubiquitin ligase, Subcellular localization, Zebrafish (Danio rerio), mRNA expression
- MeSH Terms
-
- Aeromonas/immunology*
- Amino Acid Sequence
- Animals
- Bacterial Infections/immunology*
- Bacterial Infections/microbiology*
- Gene Expression Profiling
- HEK293 Cells
- Humans
- Intracellular Space/metabolism
- Molecular Sequence Data
- Protein Transport
- Sequence Analysis, Protein
- Subcellular Fractions/metabolism
- Ubiquitin-Protein Ligases/chemistry
- Ubiquitin-Protein Ligases/genetics
- Ubiquitin-Protein Ligases/metabolism*
- Ubiquitination
- Zebrafish/genetics
- Zebrafish/immunology*
- Zebrafish/microbiology*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 25542153 Full text @ Biochem. Biophys. Res. Commun.
Citation
Zhang, X., Zhao, H., Chen, Y., Luo, H., Yang, P., Yao, B. (2015) A zebrafish (Danio rerio) bloodthirsty member 20 with E3 ubiquitin ligase activity involved in immune response against bacterial infection. Biochemical and Biophysical Research Communications. 457(1):83-9.
Abstract
The tripartite motif (TRIM)-containing proteins exhibit various activities and play important roles in the immune system through regulating signaling pathways. Bloodthirsty gene is a multigene subset of TRIM genes. In this study we identified and characterized a new member of the bloodthirsty subset of TRIM genes, btr20, in zebrafish (Danio rerio). The gene is located on chromosome 19 and forms a cluster with btr18, btr21, btr22 and an E3 ubiquitin ligase TRIM39-like gene. Deduced btr20 represents a RBCC-B30.2 TRIM protein containing 544 amino acids. The mRNA expression level of btr20 was highest in intestine and gill, followed by in spleen and kidney. Challenge experiment with Aeromonas hydrophila strain NJ-1 showed that the levels of btr20 and NF-κB mRNA were remarkably upregulated in the four tissues mentioned above. btr20 was localized in the cytoplasm and formed aggregate in human embryonic kidney cell line 293T. In vitro self-ubiquitylation experiment demonstrated that btr20 has E3 ubiquitin ligase activity that can be self-ubiquitylated with most E2 enzymes, especially UbcH6. The results suggested that btr20 may involve in the anti-microbial activity in the immune system as an E3 ubiquitin ligase.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping