PUBLICATION
Zebrafish liver (ZFL) cells are able to mount an anti-viral response after stimulation with Poly (I:C)
- Authors
- Ruyra, A., Torrealba, D., Morera, D., Tort, L., MacKenzie, S., Roher, N.
- ID
- ZDB-PUB-141224-3
- Date
- 2015
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 182: 55-63 (Journal)
- Registered Authors
- Keywords
- PAMP, ZFL, immune response, microarray, transfection, zebrafish cell line
- MeSH Terms
-
- Adaptive Immunity*
- Animals
- Cell Line
- Hepatocytes/immunology*
- Liver/cytology
- Liver/immunology*
- Poly I-C/metabolism*
- Viruses/metabolism
- Zebrafish/immunology*
- Zebrafish/virology
- PubMed
- 25536407 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Ruyra, A., Torrealba, D., Morera, D., Tort, L., MacKenzie, S., Roher, N. (2015) Zebrafish liver (ZFL) cells are able to mount an anti-viral response after stimulation with Poly (I:C). Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 182:55-63.
Abstract
The zebrafish (Danio rerio) is a widely used model species for biomedical research and is also starting to be a model for aquaculture research. The ZFL cell line, established from zebrafish liver, has been mostly used in toxicological and ecotoxicological studies. However, no studies have previously characterised this cell line in regard of its immunological response. The aim of this work was to study the gene expression response of the ZFL cell line after incubation with different prototypical immune stimuli, such as lipopolysaccharide (LPS), peptidoglycan (PGN), zymosan, and with a special focus on the dsRNA Poly (I:C). Using PCR, microarrays, and confocal microscopy we have explored the response of the ZFL cells against Poly (I:C). This study shows that the ZFL are able to uptake very efficiently the Poly (I:C) and mount a strong anti-viral response. We can conclude that ZFL could be used not only in toxicological studies, but also to study anti-viral responses in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping