PUBLICATION

miRNAs Expression Profile in Zebrafish Developing Vessels

Authors
Ristori, E., Nicoli, S.
ID
ZDB-PUB-141204-8
Date
2015
Source
Methods in molecular biology (Clifton, N.J.)   1214: 129-50 (Chapter)
Registered Authors
Nicoli, Stefania
Keywords
none
MeSH Terms
  • Animals
  • Blood Vessels/cytology
  • Blood Vessels/embryology*
  • Blood Vessels/metabolism*
  • DNA, Complementary/biosynthesis
  • DNA, Complementary/genetics
  • Endothelial Cells/metabolism
  • Gene Expression Profiling/methods*
  • Green Fluorescent Proteins/genetics
  • High-Throughput Nucleotide Sequencing
  • MicroRNAs/genetics*
  • Polymerase Chain Reaction
  • Sequence Analysis, RNA
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed
25468601 Full text @ Meth. Mol. Biol.
Abstract
In this chapter we will describe in detail a method to identify the expression profile of miRNAs in developing vessels in zebrafish embryonic development using Illumina deep sequencing strategy. We will describe how to obtain RNA from FACS-sorted primary endothelial cells from growing vessels at early stages of development and how to prepare high-quality small RNA libraries using the TruSeq small RNA strategy for Illumina Hi-Seq machine. This methodology can be applied to discover and profile all forms of small noncoding RNA, including novel miRNA and sequence variants as well as quantification of miRNAs differentially expressed in endothelial cells during angiogenesis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping