|ZFIN ID: ZDB-PUB-141203-66|
Characterization of surface phenotypic molecules of teleost dendritic cells
Shao, T., Zhu, L.Y., Nie, L., Shi, W., Dong, W.R., Xiang, L.X., Shao, J.Z.
|Source:||Developmental and comparative immunology 49: 38-43 (Journal)|
|Keywords:||Antigen presentation, Co-stimulatory signals, Surface phenotypic molecules, Zebrafish dendritic cells|
|PubMed:||25445909 Full text @ Dev. Comp. Immunol.|
Shao, T., Zhu, L.Y., Nie, L., Shi, W., Dong, W.R., Xiang, L.X., Shao, J.Z. (2015) Characterization of surface phenotypic molecules of teleost dendritic cells. Developmental and comparative immunology. 49:38-43.
ABSTRACTDendritic cells (DCs) are among the most important professional antigen-presenting cells (APCs) that participate in various biological activities in mammals. However, evidence of the existence of DCs in teleost fish and other lower vertebrates remains limited. In this study, phenotypic and functional characteristics of teleost DCs were described in a zebrafish model. An improved method to efficiently enrich DCs was established. Immunofluorescence staining revealed that the surface phenotypic hallmarks of mammalian DCs, including MHC-II, CD80/86, CD83, and CD209, were distributed on the surfaces of zebrafish DCs (DrDCs). Functional analysis results showed that DrDCs could initiate antigen-specific CD4(+) T cell activation, in which MHC-II, CD80/86, CD83, and CD209 are implicated. Hence, teleost DCs exhibit conserved immunophenotypes and functions similar to those of their mammalian counterparts. Our findings contributed to the current understanding of the evolutionary history of DCs and the DC-regulatory mechanisms of adaptive immunity.