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ZFIN ID: ZDB-PUB-141203-62
Expression and functions of ASIC1 in the zebrafish retina
Liu, S., Wang, M.X., Mao, C.J., Cheng, X.Y., Wang, C.T., Huang, J., Zhong, Z.M., Hu, W.D., Wang, F., Hu, L.F., Wang, H., Liu, C.F.
Date: 2014
Source: Biochemical and Biophysical Research Communications   455(3-4): 353-7 (Journal)
Registered Authors: Wang, Han, Zhong, Zhaomin
Keywords: Acid sensing ionic channels, Retina, Zebrafish
MeSH Terms:
  • Acid Sensing Ion Channels/genetics
  • Acid Sensing Ion Channels/physiology*
  • Animals
  • Gene Expression Regulation, Developmental
  • Immunohistochemistry
  • In Situ Hybridization
  • Larva
  • Light
  • Microscopy, Fluorescence
  • Motor Activity
  • Patch-Clamp Techniques
  • RNA, Messenger/metabolism
  • Retina/metabolism*
  • Retinal Ganglion Cells/cytology
  • Zebrafish/genetics*
  • Zebrafish/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed: 25446097 Full text @ Biochem. Biophys. Res. Commun.
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ABSTRACT
It has been demonstrated that acid sensing ionic channels (ASICs) are present in the central and peripheral nervous system of mammals, including the retina. However, it remains unclear whether the zebrafish retina also expresses ASICs. In the present study, the expression and distribution of zasic1 were examined in the retina of zebrafish. Both zasic1 mRNA and protein expressions were detected in the adult zebrafish retina. A wide distribution of ASIC1 in zebrafish retina was confirmed using whole mount in situ hybridization and immunohistochemistry study. Acidosis-induced currents in the isolated retinal ganglion cells (RGCs) were also recorded using whole cell patch clamping. Moreover, blockade of ASICs channel significantly reduced the locomotion of larval zebrafish in response to light exposure. In sum, our data demonstrate the presence of ASIC1 and its possible functional relevance in the retina of zebrafish.
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