PUBLICATION
Bioconcentration, metabolism and neurotoxicity of the organophorous flame retardant 1,3-dichloro 2-propyl phosphate (TDCPP) to zebrafish
- Authors
- Wang, Q., Lam, J.C., Man, Y.C., Lai, N.L., Kwok, K.Y., Guo, Y.Y., Lam, P.K., Zhou, B.
- ID
- ZDB-PUB-141203-20
- Date
- 2015
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 158C: 108-115 (Journal)
- Registered Authors
- Guo, YongYong, Zhou, BingSheng
- Keywords
- Bioconcentration and metabolism, Gender-specific toxicity, Neurotoxicity, TDCPP, Zebrafish
- MeSH Terms
-
- Animals
- Brain/drug effects
- Brain/metabolism
- Embryo, Nonmammalian/chemistry
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Environmental Exposure
- Female
- Flame Retardants/analysis
- Flame Retardants/metabolism*
- Flame Retardants/toxicity*
- Gene Expression Regulation/drug effects
- Male
- Nervous System/drug effects*
- Nervous System/metabolism
- Organophosphorus Compounds/analysis
- Organophosphorus Compounds/metabolism*
- Organophosphorus Compounds/toxicity*
- RNA, Messenger/genetics
- Sex Factors
- Water Pollutants, Chemical/analysis
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology
- Zebrafish/metabolism
- Zebrafish/physiology*
- PubMed
- 25461749 Full text @ Aquat. Toxicol.
- CTD
- 25461749
Citation
Wang, Q., Lam, J.C., Man, Y.C., Lai, N.L., Kwok, K.Y., Guo, Y.Y., Lam, P.K., Zhou, B. (2015) Bioconcentration, metabolism and neurotoxicity of the organophorous flame retardant 1,3-dichloro 2-propyl phosphate (TDCPP) to zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 158C:108-115.
Abstract
Organophosphate flame retardants are ubiquitous environmental contaminants; however, knowledge is limited regarding their environmental health risks and toxicity. Here, we investigated the effects of acute and long-term exposure to tris(1,3-dichloro-2-propyl) phosphate (TDCPP) to the nervous system of zebrafish. Zebrafish embryos (2h post-fertilization) were exposed to TDCPP (0-100μg/L) for 6 months up until sexual maturation. Concentrations of TDCPP and its metabolic product (bis(1,3-dichloro-2-propyl) phosphate, BDCPP) were measured in the tissues of 5 day post-fertilization (dpf) larvae. There was no effect on locomotion, acetylcholinesterase activity, levels of the neurotransmitters dopamine and serotonin, and expression of mRNAs and proteins related to central nervous system development (e.g., myelin basic protein, α1-tubulin) in any exposure group. However, in adult fish, reductions of dopamine and serotonin levels were detected in the brains of females but not males. Downregulation of nervous system development genes was observed in both the male and female brain tissues. TDCPP concentrations were measured in adult fish tissues including the brain, and greater levels were detected in females. Our results showed that females are more sensitive to TDCPP stress than males in terms of TDCPP-induced neurotoxicity. We demonstrate that long-term exposure to lower concentrations of TDCPP in fish can lead to neurotoxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping