PUBLICATION
Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation
- Authors
- Tzung, K.W., Goto, R., Saju, J.M., Sreenivasan, R., Saito, T., Arai, K., Yamaha, E., Hossain, M.S., Calvert, M.E., Orbán, L.
- ID
- ZDB-PUB-141202-8
- Date
- 2015
- Source
- Stem Cell Reports 4(1): 61-73 (Journal)
- Registered Authors
- Saju, Jolly M., Sreenivasan, Rajini
- Keywords
- none
- Datasets
- GEO:GSE57046
- MeSH Terms
-
- Models, Biological
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Cluster Analysis
- Female
- Transcriptome
- Time Factors
- Sex Characteristics
- Signal Transduction
- Transforming Growth Factor beta/metabolism
- Sex Differentiation
- Germ Cells/cytology*
- Germ Cells/metabolism*
- Meiosis
- Gene Expression Profiling
- Cell Proliferation
- Embryo, Nonmammalian
- Male
- Larva
- Animals
- Cell Differentiation
- Gonads/cytology
- Gonads/embryology*
- Gonads/metabolism*
- Reproducibility of Results
- PubMed
- 25434820 Full text @ Stem Cell Reports
Citation
Tzung, K.W., Goto, R., Saju, J.M., Sreenivasan, R., Saito, T., Arai, K., Yamaha, E., Hossain, M.S., Calvert, M.E., Orbán, L. (2015) Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation. Stem Cell Reports. 4(1):61-73.
Abstract
As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation. We applied morpholino-based gene knockdown and cell transplantation strategies to demonstrate that a threshold number of PGCs is required for the stability of ovarian fate. Using histology and transcriptomic analyses, we determined that zebrafish gonads are in a meiotic ovarian stage at 14 days postfertilization and identified signaling pathways supporting meiotic oocyte differentiation and eventual female fate. The development of PGC-depleted gonads appears to be restrained and delayed, suggesting that PGC number may directly regulate the variability and length of gonadal transformation and testicular differentiation in zebrafish. We propose that gonadal transformation may function as a developmental buffering mechanism to ensure the reproductive outcome.
Errata / Notes
Erratum: Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping