PUBLICATION

Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation

Authors
Tzung, K.W., Goto, R., Saju, J.M., Sreenivasan, R., Saito, T., Arai, K., Yamaha, E., Hossain, M.S., Calvert, M.E., Orbán, L.
ID
ZDB-PUB-141202-8
Date
2015
Source
Stem Cell Reports   4(1): 61-73 (Journal)
Registered Authors
Saju, Jolly M., Sreenivasan, Rajini
Keywords
none
Datasets
GEO:GSE57046
MeSH Terms
  • Sex Characteristics
  • Female
  • Transcriptome
  • Germ Cells/cytology*
  • Germ Cells/metabolism*
  • Cell Proliferation
  • Models, Biological
  • Cluster Analysis
  • Gonads/cytology
  • Gonads/embryology*
  • Gonads/metabolism*
  • Meiosis
  • Signal Transduction
  • Animals
  • Larva
  • Reproducibility of Results
  • Male
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Gene Expression Profiling
  • Time Factors
  • Cell Differentiation
  • Transforming Growth Factor beta/metabolism
  • Sex Differentiation
  • Embryo, Nonmammalian
(all 26)
PubMed
25434820 Full text @ Stem Cell Reports
Abstract
As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation. We applied morpholino-based gene knockdown and cell transplantation strategies to demonstrate that a threshold number of PGCs is required for the stability of ovarian fate. Using histology and transcriptomic analyses, we determined that zebrafish gonads are in a meiotic ovarian stage at 14 days postfertilization and identified signaling pathways supporting meiotic oocyte differentiation and eventual female fate. The development of PGC-depleted gonads appears to be restrained and delayed, suggesting that PGC number may directly regulate the variability and length of gonadal transformation and testicular differentiation in zebrafish. We propose that gonadal transformation may function as a developmental buffering mechanism to ensure the reproductive outcome.
Errata / Notes
Erratum: Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
slc24a5_unspecified
    		Unspecified
    zf13TgTransgenic Insertion
      zf45TgTransgenic Insertion
        zf117TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          dnd1MO1-dnd1MRPHLNO
          1 - 1 of 1
          Show
          Fish
          Fish
          zf45Tg
          zf117Tg; zf13Tg
          1 - 2 of 2
          Show
          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          DsRed2EFGDsRed2
          EGFPEFGEGFP
          GFPEFGGFP
          1 - 3 of 3
          Show
          Mapping
          No data available
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          Citation
          Tzung, K.W., Goto, R., Saju, J.M., Sreenivasan, R., Saito, T., Arai, K., Yamaha, E., Hossain, M.S., Calvert, M.E., Orbán, L. (2015) Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation. Stem Cell Reports. 4(1):61-73.