PUBLICATION
The small molecule probe PT-Yellow labels the renal proximal tubules in zebrafish
- Authors
- Sander, V., Patke, S., Sahu, S., Teoh, C.L., Peng, Z., Chang, Y.T., Davidson, A.J.
- ID
- ZDB-PUB-141120-4
- Date
- 2015
- Source
- Chemical communications (Cambridge, England) 51(2): 395-8 (Journal)
- Registered Authors
- Davidson, Alan
- Keywords
- none
- MeSH Terms
-
- Animals
- Fluorescent Dyes/administration & dosage
- Fluorescent Dyes/analysis*
- Kidney/anatomy & histology
- Kidney/ultrastructure*
- Kidney Tubules, Proximal/anatomy & histology
- Kidney Tubules, Proximal/ultrastructure*
- Larva/anatomy & histology
- Larva/ultrastructure
- Optical Imaging
- Zebrafish/anatomy & histology*
- Zebrafish/growth & development
- PubMed
- 25407666 Full text @ Chem. Commun. (Camb.)
Citation
Sander, V., Patke, S., Sahu, S., Teoh, C.L., Peng, Z., Chang, Y.T., Davidson, A.J. (2015) The small molecule probe PT-Yellow labels the renal proximal tubules in zebrafish. Chemical communications (Cambridge, England). 51(2):395-8.
Abstract
We report the development of a small fluorescent molecule, BDNCA3-D2, herein referred to as PT-Yellow. Soaking zebrafish embryos in PT-Yellow or intraperitoneal injection into adults results in non-toxic in vivo fluorescent labeling of the renal proximal tubules, the major site of blood filtrate reabsorption and a common target of injury in acute kidney injury. We demonstrate the applicability of this new compound as a rapid and simple readout for zebrafish kidney filtration and proximal tubule reabsorption function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping