PUBLICATION
Behavioral and Proteomic Analysis of Stress Response in Zebrafish (Danio rerio)
- Authors
- Magdeldin, S., Blaser, R., Yamamoto, T., Yates, J.R.
- ID
- ZDB-PUB-141116-3
- Date
- 2015
- Source
- Journal of Proteome Research 14(2): 943-52 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Behavior, Animal/physiology*
- Electroshock
- Female
- Male
- Proteome/analysis*
- Proteome/physiology
- Proteomics/methods
- Stress, Physiological/physiology*
- Stress, Psychological/physiopathology*
- Zebrafish/physiology*
- PubMed
- 25398274 Full text @ J. Proteome Res.
Citation
Magdeldin, S., Blaser, R., Yamamoto, T., Yates, J.R. (2015) Behavioral and Proteomic Analysis of Stress Response in Zebrafish (Danio rerio). Journal of Proteome Research. 14(2):943-52.
Abstract
The purpose of this study is to determine the behavioral and proteomic consequences of shock-induced stress in zebrafish (Danio rerio) as a vertebrate model. Here, we describe the behavioral effects of exposure to predictable and unpredictable electric shock, together with quantitative Tandem Mass Tag (TMT) isobaric labeling workflow in order to detect altered protein candidates in response to shock exposure. Behavioral results demonstrate a hyperactivity response to electric shock, and a suppression of activity to a stimulus predicting shock. Based on the quantitative changes in protein abundance following shock exposure, 8 proteins were significantly up-regulated (HADHB, hspa8, hspa5, actb1, mych4, atp2a1, zgc:86709, and zgc:86725). These proteins contribute crucially in catalytic activities, stress response, cation transport, and motor activities. This behavioral- proteomic driven study clearly showed that beside the rapid induction of heat shock proteins, other catalytic enzymes and cation transporters were rapidly elevated as a mechanism to counteract oxidative stress conditions resulting from elevated fear/anxiety levels.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping