PUBLICATION

Fishing on chips: Up-and-coming technological advances in analysis of zebrafish and Xenopus embryos

Authors
Zhu, F., Skommer, J., Huang, Y., Akagi, J., Adams, D., Levin, M., Hall, C.J., Crosier, P.S., Wlodkowic, D.
ID
ZDB-PUB-141008-6
Date
2014
Source
Cytometry. Part A : the journal of the International Society for Analytical Cytology   85(11): 921-32 (Review)
Registered Authors
Crosier, Phil, Hall, Chris
Keywords
FET, FETAX, Xenopus, bioanalysis, drugs, fish embryo test, lab-on-a-chip, laboratory automation, microfluidics, zebrafish
MeSH Terms
  • Animals
  • Automation, Laboratory/methods
  • Biological Assay/methods
  • Embryo Culture Techniques
  • Microfluidic Analytical Techniques/methods*
  • Xenopus/embryology
  • Zebrafish/embryology*
PubMed
25287981 Full text @ Cytometry A
Abstract
Biotests performed on small vertebrate model organisms provide significant investigative advantages as compared with bioassays that employ cell lines, isolated primary cells, or tissue samples. The main advantage offered by whole-organism approaches is that the effects under study occur in the context of intact physiological milieu, with all its intercellular and multisystem interactions. The gap between the high-throughput cell-based in vitro assays and low-throughput, disproportionally expensive and ethically controversial mammal in vivo tests can be closed by small model organisms such as zebrafish or Xenopus. The optical transparency of their tissues, the ease of genetic manipulation and straightforward husbandry, explain the growing popularity of these model organisms. Nevertheless, despite the potential for miniaturization, automation and subsequent increase in throughput of experimental setups, the manipulation, dispensing and analysis of living fish and frog embryos remain labor-intensive. Recently, a new generation of miniaturized chip-based devices have been developed for zebrafish and Xenopus embryo on-chip culture and experimentation. In this work, we review the critical developments in the field of Lab-on-a-Chip devices designed to alleviate the limits of traditional platforms for studies on zebrafish and clawed frog embryo and larvae.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes