PUBLICATION
Involvement of zebrafish RIG-I in NF-?B and IFN signaling pathways: Insights into functional conservation of RIG-I in antiviral innate immunity
- Authors
- Nie, L., Zhang, Y.S., Dong, W.R., Xiang, L.X., Shao, J.Z.
- ID
- ZDB-PUB-140930-1
- Date
- 2015
- Source
- Developmental and comparative immunology 48(1): 95-101 (Journal)
- Registered Authors
- Keywords
- IFN signaling pathway, NF-?B signaling pathway, TRIM25 regulation, zebrafish RIG-I
- MeSH Terms
-
- Antiviral Agents/metabolism
- Molecular Sequence Data
- CARD Signaling Adaptor Proteins/biosynthesis
- CARD Signaling Adaptor Proteins/metabolism
- Transcription Factors/genetics
- Amino Acid Sequence
- Gene Knockdown Techniques
- Male
- Fish Diseases/immunology
- Female
- RNA Virus Infections/immunology*
- RNA Virus Infections/virology
- Interleukin-8/biosynthesis
- Morpholinos/genetics
- Immunity, Innate/immunology
- Myxovirus Resistance Proteins/biosynthesis
- Interferons/metabolism*
- Tumor Necrosis Factor-alpha/biosynthesis
- Zebrafish/immunology*
- Zebrafish/virology
- NF-kappa B/metabolism*
- Animals
- Sequence Alignment
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Base Sequence
- RNA Viruses/immunology
- Sequence Analysis, DNA
- DEAD-box RNA Helicases
- PubMed
- 25265425 Full text @ Dev. Comp. Immunol.
Citation
Nie, L., Zhang, Y.S., Dong, W.R., Xiang, L.X., Shao, J.Z. (2015) Involvement of zebrafish RIG-I in NF-?B and IFN signaling pathways: Insights into functional conservation of RIG-I in antiviral innate immunity. Developmental and comparative immunology. 48(1):95-101.
Abstract
The retinoic acid-inducible gene I (RIG-I) is a critical sensor for host recognition of RNA virus infection and initiation of antiviral signaling pathways in mammals. However, data on the occurrence and functions of this molecule in lower vertebrates are limited. In this study, we characterized an RIG-I homolog (DrRIG-I) from zebrafish. Structurally, this DrRIG-I shares a number of conserved functional domains/motifs with its mammalian counterparts, namely, caspase activation and recruitment domain, DExD/H box, a helicase domain, and a C-terminal domain. Functionally, stimulation with DrRIG-I CARD in zebrafish embryos significantly activated the NF-κB and IFN signaling pathways, leading to the expression of TNF-α, IL-8 and IFN-induced Mx, ISG15, and viperin. However, knockdown of TRIM25 (a pivotal activator for RIG-I receptors) significantly suppressed the induced activation of IFN signaling. Results suggested the functional conservation of RIG-I receptors in the NF-κB and IFN signaling pathways between teleosts and mammals, providing a perspective into the evolutionary history of RIG-I-mediated antiviral innate immunity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping