ZFIN ID: ZDB-PUB-140928-9
Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain
Hatef, A., Shajan, S., Unniappan, S.
Date: 2015
Source: General and comparative endocrinology   215: 51-60 (Journal)
Registered Authors: Unniappan, Suraj
Keywords: Food deprivation, Immunohistochemistry, Nucleobindin, Nutrients, Periprandial, Zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Brain/metabolism*
  • Calcium-Binding Proteins/genetics*
  • Calcium-Binding Proteins/metabolism*
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/metabolism*
  • Food*
  • Food Deprivation
  • Gastrointestinal Tract/metabolism*
  • Gene Expression Regulation*
  • Ghrelin/genetics
  • Ghrelin/metabolism
  • Immunoenzyme Techniques
  • Liver/metabolism*
  • Molecular Sequence Data
  • Nerve Tissue Proteins/genetics*
  • Nerve Tissue Proteins/metabolism*
  • Phylogeny
  • RNA, Messenger/genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 25260251 Full text @ Gen. Comp. Endocrinol.
Nesfatin-1 is a naturally occurring, 82-amino acid peptide processed from the precursor nucleobindin 2 (NUCB2), a highly conserved protein among vertebrates. In fish, two isoforms of NUCB2 (NUCB2A and NUCB2B) exist, and nesfatin-1 has been identified in goldfish and Ya fish. We recently reported the presence and appetite suppressing effects of nesfatin-1 in goldfish. The main objectives of this study were to characterize NUCB2 in zebrafish, and determine whether NUCB2 mRNAs are affected by food availability. Tissue distribution of NUCB2A and NUCB2B mRNAs, and NUCB2/nesfatin-1-like immunoreactivity (ir) in the gut of zebrafish were also investigated. In zebrafish, nesfatin-1 region (1-82 amino acids) in NUCB2A is 78% identical to NUCB2B. Both NUCB2A and NUCB2 mRNAs were most abundant in the liver, while less abundance was found in other tissues including the brain and gut. NUCB2/nesfatin-1-like immunoreactivity was detected in the mucosal layer cells of the zebrafish anterior gastrointestinal tract. NUCB2A and NUCB2B mRNA expression were decreased in the brain of zebrafish 3h after feeding, and after a 7-day food deprivation. Both NUCB2A and NUCB2B mRNAs in the gut were also decreased following 7days of food deprivation, while NUCB2B mRNA was increased in the liver. Our results provide molecular and functional evidences to support potential anorectic and metabolic roles for endogenous nesfatin-1 in zebrafish. To our knowledge, this is the first report on NUCB2B characterization in vertebrates.