PUBLICATION
Comparative genomic of the teleost cathepsin B and H and involvement in bacterial induced immunity of miiuy croaker
- Authors
- Che, R., Wang, R., Xu, T.
- ID
- ZDB-PUB-140903-1
- Date
- 2014
- Source
- Fish & shellfish immunology 41(2): 163-71 (Journal)
- Registered Authors
- Keywords
- Cathepsin, Comparative genomics, Expression pattern, Gene synteny, Immune responses, Miichthys miiuy (miiuy croaker), Teleost genome
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cathepsin B/genetics*
- Cathepsin B/immunology
- Cathepsin H/genetics*
- Cathepsin H/immunology
- Gene Expression Regulation/immunology*
- Liver/metabolism
- Molecular Sequence Data
- Perciformes/genetics*
- Perciformes/immunology
- Perciformes/microbiology*
- Phylogeny*
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Sequence Analysis, DNA
- Species Specificity
- Spleen/metabolism
- Synteny
- PubMed
- 25181651 Full text @ Fish Shellfish Immunol.
Citation
Che, R., Wang, R., Xu, T. (2014) Comparative genomic of the teleost cathepsin B and H and involvement in bacterial induced immunity of miiuy croaker. Fish & shellfish immunology. 41(2):163-71.
Abstract
Cathepsins are a family of lysosomal proteases play different roles at physiological and pathological states and present in almost all animals as well as other organisms. Cathepsins B and H are both cysteine proteases of cathepsins. Cathepsin B and H have been studied playing parts in protein degradation/turnover, antigen presentation/processing and hormone maturation in mammals. However, little is known about the structures and functions of cathepsin B and H in teleosts. In the present study, we identified and characterized the full-length miiuy croaker (Miichthys miiuy) cathepsin B and H genes. The sequence analysis results showed that both cathepsin B and H contain the characteristic of papain family with a signal peptide, propeptide and mature peptide regions. The comparison of the genomic organizations and locations indicated the conserved synteny and mild evolution in the cathepsin B and H genes adjacent regions. In addition, the gene synteny analysis showed that miiuy croaker cathepsin B has a closer relationship to stickleback and fugu than to cave fish and zebrafish, and cathepsin H was most similar with the 2 subtype in tilapia and fugu. By phylogenetic analysis, miiuy croaker cathepsin B and H were all assigned to cysteine proteases, and with a close relationship to Salmo salar cathepsin B and Oplegnathus fasciatus cathepsin H, respectively. Quantitative real-time RT-PCR analysis results confirmed that cathepsin B and H genes expressed ubiquitously in all tested healthy tissues from miiuy croaker. Furthermore, up-regulated expression of the cathepsin B and H transcripts in liver, spleen and kidney after exposure upon Vibrio anguillarum suggested that they may play important roles in innate immune response and antigen processing of miiuy croaker.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping