PUBLICATION
Nitrate (NO3(-)) and nitrite (NO2(-)) are endocrine disruptors to downregulate expression of tyrosine hydroxylase and motor behavior through conversion to nitric oxide in early development of zebrafish
- Authors
- Jannat, M., Fatimah, R., Kishida, M.
- ID
- ZDB-PUB-140901-2
- Date
- 2014
- Source
- Biochemical and Biophysical Research Communications 452(3): 608-13 (Journal)
- Registered Authors
- Kishida, Mitsuyo
- Keywords
- Dopaminergic neuron, Early development, Nitrate, Nitric oxide, Nitrite, Zebrafish
- MeSH Terms
-
- Receptors, Estrogen/antagonists & inhibitors
- Receptors, Estrogen/genetics
- Receptors, Estrogen/metabolism
- Larva/drug effects
- Larva/genetics
- Larva/metabolism
- Enzyme Inhibitors/pharmacology
- Oxidoreductases/antagonists & inhibitors
- Oxidoreductases/genetics
- Oxidoreductases/metabolism
- Gene Expression/drug effects
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Nitric Oxide/toxicity*
- Onium Compounds/pharmacology
- Nitrites/toxicity*
- Dopaminergic Neurons/drug effects*
- Dopaminergic Neurons/enzymology
- Embryo, Nonmammalian
- Water Pollutants, Chemical/toxicity
- Endocrine Disruptors/toxicity*
- Nitrates/toxicity*
- Animals
- Estradiol/analogs & derivatives
- Estradiol/pharmacology
- Tyrosine 3-Monooxygenase/antagonists & inhibitors*
- Tyrosine 3-Monooxygenase/genetics
- Tyrosine 3-Monooxygenase/metabolism
- Motor Activity/drug effects
- Estrogen Receptor Antagonists/pharmacology
- PubMed
- 25173937 Full text @ Biochem. Biophys. Res. Commun.
Citation
Jannat, M., Fatimah, R., Kishida, M. (2014) Nitrate (NO3(-)) and nitrite (NO2(-)) are endocrine disruptors to downregulate expression of tyrosine hydroxylase and motor behavior through conversion to nitric oxide in early development of zebrafish. Biochemical and Biophysical Research Communications. 452(3):608-13.
Abstract
With a view to consider the increasing concern over nitrogen pollution in the aquatic environment, we investigated effects of nitrate (NO3(-)) and nitrite (NO2(-)) on the activity of dopaminergic neuron in zebrafish embryos and larvae. Both nitrate and nitrite exposure decreased the expression of tyrosine hydroxylase (TH) in dopaminergic neurons at 48 hpf. Only nitrite decreased the response to tactile stimulation at 72 hpf, whereas both nitrate and nitrite decreased the swimming activity at 6 dpf. When the embryos were exposed to nitrate or nitrite together with an estrogen receptor blocker (ICI 182,780), the decreases in TH expression and motor behavior caused by nitrate or nitrite alone were reversed suggesting the effects of nitrate and nitrite were mediated through estrogen receptor (ER). The result of co-incubation with an oxidoreductase inhibitor, diphenyleneiodonium, indicated the conversion to nitric oxide (NO) is likely to be responsible for the effects of nitrate and nitrite, which was further supported by the increased staining for NO after exposure. The present study demonstrates that nitrate and nitrite are neurotoxicants acting as an endocrine disruptor possibly through conversion to NO to downretulate the activity of dopaminergic neuron in early development of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping