PUBLICATION
The PDZ-Containing Unconventional Myosin XVIIIA Regulates Embryonic Muscle Integrity in Zebrafish
- Authors
- Cao, J., Li, S., Shao, M., Cheng, X., Xu, Z., Shi, D.
- ID
- ZDB-PUB-140828-5
- Date
- 2014
- Source
- Journal of genetics and genomics = Yi chuan xue bao 41: 417-428 (Journal)
- Registered Authors
- Keywords
- Dystroglycan, Dystrophin, MYO18A, Muscle, Myofiber, Myosin XVIIIA, Unconventional myosin, Zebrafish
- MeSH Terms
-
- Animals
- Cell Adhesion
- Dystroglycans/metabolism
- Dystrophin/metabolism
- Dystrophin-Associated Protein Complex/metabolism
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Gene Knockdown Techniques
- Lamins/metabolism
- Muscles/cytology
- Muscles/embryology*
- Muscles/metabolism*
- Myoblasts/cytology
- Myoblasts/metabolism
- Myosins/chemistry*
- Myosins/deficiency
- Myosins/genetics
- Myosins/metabolism*
- PDZ Domains*
- Protein Transport
- Somites/cytology
- Somites/metabolism
- Zebrafish/embryology*
- PubMed
- 25160974 Full text @ J. Genet. Genomics
Citation
Cao, J., Li, S., Shao, M., Cheng, X., Xu, Z., Shi, D. (2014) The PDZ-Containing Unconventional Myosin XVIIIA Regulates Embryonic Muscle Integrity in Zebrafish. Journal of genetics and genomics = Yi chuan xue bao. 41:417-428.
Abstract
Myosin XVIIIA, or MYO18A, is a unique PDZ domain-containing unconventional myosin and is evolutionarily conserved from Drosophila to vertebrates. Although there is evidence indicating its expression in the somites, whether it regulates muscle function remains unclear. We show that the two zebrafish myo18a genes (myo18aa and myo18ab) are predominantly expressed at somite borders during early developmental stages. Knockdown of these genes or overexpression of the MYO18A PDZ domain disrupts myofiber integrity, induces myofiber lesions, and compromises the localization of dystrophin, α-dystroglycan (α-DG) and laminin at the myotome boundaries. Cell transplantation experiments indicate that myo18a morphant myoblasts fail to form elongated myofibers in the myotomes of wild-type embryos, which can be rescued by the full-length MYO18A protein. These results suggest that MYO18A likely functions in the adhesion process that maintains the stable attachment of myofibers to ECM (extracellular matrix) and muscle integrity during early development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping