ZFIN ID: ZDB-PUB-140819-4
Sequential Gene Silencing Using Wavelength-Selective Caged Morpholino Oligonucleotides
Yamazoe, S., Liu, Q., McQuade, L.E., Deiters, A., Chen, J.K.
Date: 2014
Source: Angewandte Chemie (International ed. in English)   53(38): 10114-8 (Journal)
Registered Authors: Chen, James K.
Keywords: antisense agents, cage compounds, developmental biology, gene expression, oligonucleotides
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Gene Silencing*
  • Molecular Structure
  • Morpholinos/chemistry
  • Morpholinos/genetics*
  • Zebrafish/embryology
PubMed: 25130695 Full text @ Angew. Chem. Int. Ed. Engl.
Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength-selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse-genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning.