PUBLICATION
A Self-Organizing miR-132/Ctbp2 Circuit Regulates Bimodal Notch Signals and Glial Progenitor Fate Choice during Spinal Cord Maturation
- Authors
- Salta, E., Lau, P., Sala Frigerio, C., Coolen, M., Bally-Cuif, L., De Strooper, B.
- ID
- ZDB-PUB-140819-1
- Date
- 2014
- Source
- Developmental Cell 30(4): 423-36 (Journal)
- Registered Authors
- Bally-Cuif, Laure, Coolen, Marion
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation*
- Feedback, Physiological
- Gene Expression Regulation, Developmental
- MicroRNAs/genetics
- MicroRNAs/metabolism*
- Microglia/cytology
- Microglia/metabolism*
- Neural Stem Cells/cytology
- Neural Stem Cells/metabolism*
- Receptors, Notch/genetics
- Receptors, Notch/metabolism*
- Repressor Proteins/genetics
- Repressor Proteins/metabolism*
- Sirtuin 1/genetics
- Sirtuin 1/metabolism
- Spinal Cord/cytology
- Spinal Cord/embryology
- Spinal Cord/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 25132384 Full text @ Dev. Cell
Citation
Salta, E., Lau, P., Sala Frigerio, C., Coolen, M., Bally-Cuif, L., De Strooper, B. (2014) A Self-Organizing miR-132/Ctbp2 Circuit Regulates Bimodal Notch Signals and Glial Progenitor Fate Choice during Spinal Cord Maturation. Developmental Cell. 30(4):423-36.
Abstract
Radial glial progenitors play pivotal roles in the development and patterning of the spinal cord, and their fate is controlled by Notch signaling. How Notch is shaped to regulate their crucial transition from expansion toward differentiation remains, however, unknown. miR-132 in the developing zebrafish dampens Notch signaling via a cascade involving the transcriptional corepressor Ctbp2 and the Notch suppressor Sirt1. At early embryonic stages, high Ctbp2 levels sustain Notch signaling and radial glial expansion and concomitantly induce miR-132 expression via a double-negative feedback loop involving Rest inhibition. The changing balance in miR-132 and Ctbp2 interaction gradually drives the switch in Notch output and radial glial progenitor fate as part of the larger developmental program involved in the transition from embryonic to larval spinal cord.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping