PUBLICATION

A Self-Organizing miR-132/Ctbp2 Circuit Regulates Bimodal Notch Signals and Glial Progenitor Fate Choice during Spinal Cord Maturation

Authors
Salta, E., Lau, P., Sala Frigerio, C., Coolen, M., Bally-Cuif, L., De Strooper, B.
ID
ZDB-PUB-140819-1
Date
2014
Source
Developmental Cell   30(4): 423-36 (Journal)
Registered Authors
Bally-Cuif, Laure, Coolen, Marion
Keywords
none
MeSH Terms
  • Animals
  • Cell Differentiation*
  • Feedback, Physiological
  • Gene Expression Regulation, Developmental
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Microglia/cytology
  • Microglia/metabolism*
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism*
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism*
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism*
  • Sirtuin 1/genetics
  • Sirtuin 1/metabolism
  • Spinal Cord/cytology
  • Spinal Cord/embryology
  • Spinal Cord/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
25132384 Full text @ Dev. Cell
Abstract
Radial glial progenitors play pivotal roles in the development and patterning of the spinal cord, and their fate is controlled by Notch signaling. How Notch is shaped to regulate their crucial transition from expansion toward differentiation remains, however, unknown. miR-132 in the developing zebrafish dampens Notch signaling via a cascade involving the transcriptional corepressor Ctbp2 and the Notch suppressor Sirt1. At early embryonic stages, high Ctbp2 levels sustain Notch signaling and radial glial expansion and concomitantly induce miR-132 expression via a double-negative feedback loop involving Rest inhibition. The changing balance in miR-132 and Ctbp2 interaction gradually drives the switch in Notch output and radial glial progenitor fate as part of the larger developmental program involved in the transition from embryonic to larval spinal cord.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes