PUBLICATION
Quantitative Fluorescence Ratio Imaging of Intralysosomal Chloride Ions with Single Excitation/Dual Maximum Emission
- Authors
- Li, P., Zhang, S., Fan, N., Xiao, H., Zhang, W., Zhang, W., Wang, H., Tang, B.
- ID
- ZDB-PUB-140808-8
- Date
- 2014
- Source
- Chemistry (Weinheim an der Bergstrasse, Germany) 20(37): 11760-7 (Journal)
- Registered Authors
- Wang, Hui
- Keywords
- anions, chlorine, fluorescence, imaging agents, lysosomes
- MeSH Terms
-
- Animals
- Chlorides/chemistry*
- Chlorides/metabolism
- Hep G2 Cells
- Humans
- Lysosomes/chemistry*
- Lysosomes/metabolism
- Rats
- Spectrometry, Fluorescence/methods*
- PubMed
- 25098912 Full text @ Chemistry
Citation
Li, P., Zhang, S., Fan, N., Xiao, H., Zhang, W., Zhang, W., Wang, H., Tang, B. (2014) Quantitative Fluorescence Ratio Imaging of Intralysosomal Chloride Ions with Single Excitation/Dual Maximum Emission. Chemistry (Weinheim an der Bergstrasse, Germany). 20(37):11760-7.
Abstract
Fluorescence ratio imaging is currently being used to quantitatively detect biologically active molecules in biosystems; however, two excitations of most existing fluorescent ratiometric probes account for cumbersome operating conditions for imaging. Thus, a fluorescent ratiometric probe, 6-methoxyquinolinium-dansyl (MQ-DS), for Cl(-) with single excitation/dual maximum emission has been developed. MQ-DS can preferably localize into lysosomes and display excellent photostability. Upon excitation at a single wavelength, it responds precisely and instantaneously to changes in Cl(-) concentrations, and it can be conveniently utilized to implement real-time fluorescence ratio imaging to quantitatively track alterations in Cl(-) levels inside cells treated under various pH conditions, and also in zebrafish with acute wounds. The successful application of the new probe in bioimaging may greatly facilitate a complete understanding of the physiological functions of Cl(-) .
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping