PUBLICATION
Role of the ANKMY2-FKBP38 Axis in Regulation of the Sonic Hedgehog (Shh) Signaling Pathway
- Authors
- Saita, S., Shirane, M., Ishitani, T., Shimizu, N., Nakayama, K.I.
- ID
- ZDB-PUB-140801-15
- Date
- 2014
- Source
- The Journal of biological chemistry 289(37): 25639-54 (Journal)
- Registered Authors
- Ishitani, Tohru, Shimizu, Nobuyuki
- Keywords
- Hedgehog signaling pathway, cell signaling, mouse, prolyl isomerase, proteomics, zebrafish
- MeSH Terms
-
- Animals
- Carrier Proteins/genetics*
- Gene Expression Regulation, Developmental
- Hedgehog Proteins/genetics
- Hedgehog Proteins/metabolism*
- Mice
- Signal Transduction/genetics*
- Tacrolimus Binding Proteins/genetics
- Tacrolimus Binding Proteins/metabolism*
- Trans-Activators/metabolism
- Zebrafish/genetics
- PubMed
- 25077969 Full text @ J. Biol. Chem.
Citation
Saita, S., Shirane, M., Ishitani, T., Shimizu, N., Nakayama, K.I. (2014) Role of the ANKMY2-FKBP38 Axis in Regulation of the Sonic Hedgehog (Shh) Signaling Pathway. The Journal of biological chemistry. 289(37):25639-54.
Abstract
Sonic hedgehog (Shh) is a secreted morphogen that controls the patterning and growth of various tissues in the developing vertebrate embryo, including the central nervous system. Ablation of the FK506-binding protein 38 (FKBP38) gene results in activation of the Shh signaling pathway in mouse embryos, but the molecular mechanism by which FKBP38 suppresses Shh signaling has remained unclear. With the use of a proteomics approach, we have now identified ANKMY2, a protein with three ankyrin repeats and a MYND (myeloid, Nervy, and DEAF-1)-type Zn(2+)-finger domain, as a molecule that interacts with FKBP38. Co-immunoprecipitation analysis confirmed that endogenous FKBP38 and ANKMY2 interact in mouse brain. Depletion or overexpression of ANKMY2 resulted in down- and up-regulation of Shh signaling, respectively, in mouse embryonic fibroblasts. Furthermore, combined depletion of both FKBP38 and ANKMY2 attenuated Shh signaling in these cells, suggesting that ANKMY2 acts downstream of FKBP38 to activate the Shh signaling pathway. Targeting of the zebrafish ortholog of mouse Ankmy2 (ankmy2a) in fish embryos with an antisense morpholino oligonucleotide conferred a phenotype reflecting loss of function of the Shh pathway, suggesting that the regulation of Shh signaling by ANKMY2 is conserved between mammals and fish. Our findings thus indicate that the FKBP38-ANKMY2 axis plays a key role in regulation of Shh signaling in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping