PUBLICATION
Myomaker mediates fusion of fast myocytes in zebrafish embryos
- Authors
- Landemaine, A., Rescan, P.Y., Gabillard, J.C.
- ID
- ZDB-PUB-140801-12
- Date
- 2014
- Source
- Biochemical and Biophysical Research Communications 451(4): 480-4 (Journal)
- Registered Authors
- Keywords
- Fast myotome, Fusion, Muscle growth, Myocyte, Zebrafish
- MeSH Terms
-
- Animals
- Cell Fusion
- Gene Expression Regulation, Developmental
- Membrane Proteins/biosynthesis*
- Mice
- Muscle Cells/physiology*
- Muscle Development/genetics
- Muscle Proteins/biosynthesis*
- Zebrafish/embryology
- PubMed
- 25078621 Full text @ Biochem. Biophys. Res. Commun.
Citation
Landemaine, A., Rescan, P.Y., Gabillard, J.C. (2014) Myomaker mediates fusion of fast myocytes in zebrafish embryos. Biochemical and Biophysical Research Communications. 451(4):480-4.
Abstract
Myomaker (also called Tmem8c), a new membrane activator of myocyte fusion was recently discovered in mice. Using whole mount in situ hybridization on zebrafish embryos at different stages of embryonic development, we show that myomaker is transiently expressed in fast myocytes forming the bulk of zebrafish myotome. Zebrafish embryos injected with morpholino targeted against myomaker were alive after yolk resorption and appeared morphologically normal, but they were unable to swim, even under effect of a tactile stimulation. Confocal observations showed a marked phenotype characterized by the persistence of mononucleated muscle cells in the fast myotome at developmental stages where these cells normally fuse to form multinucleated myotubes. This indicates that myomaker is essential for myocyte fusion in zebrafish. Thus, there is an evolutionary conservation of myomaker expression and function among Teleostomi.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping