PUBLICATION

In vivo imaging of hedgehog pathway activation with a nuclear fluorescent reporter

Authors
Mich, J.K., Payumo, A.Y., Rack, P.G., Chen, J.K.
ID
ZDB-PUB-140730-10
Date
2014
Source
PLoS One   9: e103661 (Journal)
Registered Authors
Chen, James K., Mich, John, Rack, Paul
Keywords
none
MeSH Terms
  • Mutation
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Animal Fins/embryology
  • Animal Fins/growth & development
  • Animal Fins/metabolism
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism*
  • Animals, Genetically Modified
  • Cell Nucleus/metabolism*
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Signal Transduction*
  • Microscopy, Fluorescence
  • Time-Lapse Imaging/methods
  • Reproducibility of Results
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Animals
  • Somites/embryology
  • Somites/growth & development
  • Somites/metabolism
(all 25)
PubMed
25068273 Full text @ PLoS One
Abstract
The Hedgehog (Hh) pathway is essential for embryonic development and tissue regeneration, and its dysregulation can lead to birth defects and tumorigenesis. Understanding how this signaling mechanism contributes to these processes would benefit from an ability to visualize Hedgehog pathway activity in live organisms, in real time, and with single-cell resolution. We report here the generation of transgenic zebrafish lines that express nuclear-localized mCherry fluorescent protein in a Gli transcription factor-dependent manner. As demonstrated by chemical and genetic perturbations, these lines faithfully report Hedgehog pathway state in individual cells and with high detection sensitivity. They will be valuable tools for studying dynamic Gli-dependent processes in vertebrates and for identifying new chemical and genetic regulators of the Hh pathway.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
Df(Chr07)t4/t4
dzip1tm79a/tm79a
gli1ts269/ts269
sb15Tg
smohi1640Tg/hi1640Tg
st1001Tg
st1002Tg
1 - 7 of 7
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Antibodies
No data available
Orthology
Engineered Foreign Genes
Marker Marker Type Name
GFPEFGGFP
mCherryEFGmCherry
1 - 2 of 2
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Mapping
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Citation
Mich, J.K., Payumo, A.Y., Rack, P.G., Chen, J.K. (2014) In vivo imaging of hedgehog pathway activation with a nuclear fluorescent reporter. PLoS One. 9:e103661.