PUBLICATION

Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio)

Authors
Wang, Q., Tan, X., Jiao, S., You, F., Zhang, P.J.
ID
ZDB-PUB-140728-16
Date
2014
Source
PLoS One   9: e102492 (Journal)
Registered Authors
Keywords
none
Datasets
GEO:GSE58988
MeSH Terms
  • Animals
  • Promoter Regions, Genetic
  • Animals, Genetically Modified*
  • Adenosine Triphosphate/metabolism
  • Carps/genetics
  • Carps/metabolism
  • Circadian Rhythm/genetics
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Biological Transport
  • Muscle, Skeletal/metabolism*
  • Molecular Sequence Annotation
  • Histone-Lysine N-Methyltransferase/genetics
  • Histone-Lysine N-Methyltransferase/metabolism
  • Cold Temperature
  • Energy Metabolism/genetics
  • Lipid Metabolism
  • Transgenes
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Gene Expression Profiling
  • Creatine Kinase, MM Form/genetics
  • Creatine Kinase, MM Form/metabolism
  • Acclimatization/genetics*
  • Gene Expression Regulation*
(all 25)
PubMed
25058652 Full text @ PLoS One
Abstract
Low temperatures may cause severe growth inhibition and mortality in fish. In order to understand the mechanism of cold tolerance, a transgenic zebrafish Tg (smyd1:m3ck) model was established to study the effect of energy homeostasis during cold stress. The muscle-specific promoter Smyd1 was used to express the carp muscle form III of creatine kinase (M3-CK), which maintained enzymatic activity at a relatively low temperature, in zebrafish skeletal muscle. In situ hybridization showed that M3-CK was expressed strongly in the skeletal muscle. When exposed to 13°C, Tg (smyd1:m3ck) fish maintained their swimming behavior, while the wild-type could not. Energy measurements showed that the concentration of ATP increased in Tg (smyd1:m3ck) versus wild-type fish at 28°C. After 2 h at 13°C, ATP concentrations were 2.16-fold higher in Tg (smyd1:m3ck) than in wild-type (P<0.05). At 13°C, the ATP concentration in Tg (smyd1:m3ck) fish and wild-type fish was 63.3% and 20.0%, respectively, of that in wild-type fish at 28°C. Microarray analysis revealed differential expression of 1249 transcripts in Tg (smyd1:m3ck) versus wild-type fish under cold stress. Biological processes that were significantly overrepresented in this group included circadian rhythm, energy metabolism, lipid transport, and metabolism. These results are clues to understanding the mechanisms underlying temperature acclimation in fish.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf547TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    zf547Tg
    1 - 1 of 1
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
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    Citation
    Wang, Q., Tan, X., Jiao, S., You, F., Zhang, P.J. (2014) Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio). PLoS One. 9:e102492.