PUBLICATION

Optimized cell transplantation using adult rag2 mutant zebrafish

Authors
Tang, Q., Abdelfattah, N.S., Blackburn, J.S., Moore, J.C., Martinez, S.A., Moore, F.E., Lobbardi, R., Tenente, I.M., Ignatius, M.S., Berman, J.N., Liwski, R.S., Houvras, Y., Langenau, D.M.
ID
ZDB-PUB-140722-20
Date
2014
Source
Nature Methods   11(8): 821-4 (Journal)
Registered Authors
Berman, Jason, Houvras, Yariv, Ignatius, Myron, Langenau, David, Moore, John
Keywords
none
MeSH Terms
  • Aged
  • Animals
  • Cell Transplantation*
  • DNA-Binding Proteins/genetics*
  • Humans
  • Mutation*
  • Zebrafish/genetics*
PubMed
25042784 Full text @ Nat. Methods
Abstract
Cell transplantation into adult zebrafish has lagged behind mouse models owing to the lack of immunocompromised strains. Here we have created rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft muscle, blood stem cells and various cancers. rag2(E450fs) mutant zebrafish are the first immunocompromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes