ZFIN ID: ZDB-PUB-140722-18
High-resolution reconstruction of the beating zebrafish heart
Mickoleit, M., Schmid, B., Weber, M., Fahrbach, F.O., Hombach, S., Reischauer, S., Huisken, J.
Date: 2014
Source: Nature Methods 11(9): 919-22 (Journal)
Registered Authors: Hombach, Sonja, Huisken, Jan, Mickoleit, Michaela, Reischauer, Sven
Keywords: none
MeSH Terms:
  • Algorithms
  • Animals
  • Cell Tracking/methods*
  • Image Enhancement/methods*
  • Image Interpretation, Computer-Assisted/methods*
  • Imaging, Three-Dimensional/methods*
  • Microscopy, Video/methods*
  • Myocytes, Cardiac/cytology*
  • Myocytes, Cardiac/physiology
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Subtraction Technique
  • Zebrafish/anatomy & histology*
  • Zebrafish/physiology
PubMed: 25042787 Full text @ Nat. Methods
ABSTRACT
The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.
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