Mickoleit, M., Schmid, B., Weber, M., Fahrbach, F.O., Hombach, S., Reischauer, S., Huisken, J. (2014) High-resolution reconstruction of the beating zebrafish heart. Nature Methods. 11(9):919-22.
The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.