ZFIN ID: ZDB-PUB-140712-12
Clonal Analysis of kit ligand a Functional Expression Reveals Lineage-Specific Competence to Promote Melanocyte Rescue in the Mutant Regenerating Caudal Fin
Tryon, R.C., Johnson, S.L.
Date: 2014
Source: PLoS One   9: e102317 (Journal)
Registered Authors: Johnson, Stephen L.
Keywords: none
MeSH Terms:
  • Animal Fins/growth & development*
  • Animals
  • Cell Differentiation/genetics*
  • Cell Lineage/genetics
  • Fibroblasts/cytology
  • Gene Expression Regulation, Developmental
  • Melanocytes/metabolism
  • Osteoblasts/cytology
  • Promoter Regions, Genetic
  • Regeneration/genetics*
  • Stem Cell Factor/biosynthesis*
  • Stem Cell Factor/genetics
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
PubMed: 25009992 Full text @ PLoS One
The study of regeneration in an in vivo vertebrate system has the potential to reveal targetable genes and pathways that could improve our ability to heal and repair damaged tissue. We have developed a system for clonal labeling of discrete cell lineages and independently inducing gene expression under control of the heat shock promoter in the zebrafish caudal fin. Consequently we are able to test the affects of overexpressing a single gene in the context of regeneration within each of the nine different cell lineage classes that comprise the caudal fin. This can test which lineage is necessary or sufficient to provide gene function. As a first example to demonstrate this approach, we explored which lineages were competent to functionally express the kit ligand a protein as assessed by the local complementation of the mutation in the sparse-like (kitlgatc244b) background. We show that dermal fibroblast expression of kit ligand a robustly supports the rescue of melanocytes in the regenerating caudal fin. kit ligand a expression from skin and osteoblasts results in more modest and variable rescue of melanocytes, while lateral line expression was unable to complement the mutation.