PUBLICATION
Mammalian Nkx2.2(+) perineurial glia are essential for motor nerve development
- Authors
- Clark, J.K., O'Keefe, A., Mastracci, T.L., Sussel, L., Matise, M.P., Kucenas, S.
- ID
- ZDB-PUB-140701-1
- Date
- 2014
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 243(9): 1116-29 (Journal)
- Registered Authors
- Kucenas, Sarah
- Keywords
- Schwann cell, motor nerve, perineurial glia, perineurium, peripheral nervous system, perisynaptic Schwann cell
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axons/physiology
- Cell Differentiation/physiology*
- Cell Line
- Homeodomain Proteins/metabolism*
- Mice
- Motor Neurons/cytology*
- Motor Neurons/metabolism
- Neuroglia/cytology*
- Neuroglia/metabolism
- Peripheral Nerves/cytology*
- Peripheral Nerves/metabolism
- Schwann Cells/cytology*
- Schwann Cells/metabolism
- Transcription Factors/metabolism*
- PubMed
- 24979729 Full text @ Dev. Dyn.
Citation
Clark, J.K., O'Keefe, A., Mastracci, T.L., Sussel, L., Matise, M.P., Kucenas, S. (2014) Mammalian Nkx2.2(+) perineurial glia are essential for motor nerve development. Developmental Dynamics : an official publication of the American Association of Anatomists. 243(9):1116-29.
Abstract
Background All vertebrate peripheral nerves connect the central nervous system (CNS) with targets in the periphery and are composed of axons, layers of ensheathing glia and connective tissue. Although the structure of these conduits is well established, very little is known about the origin and developmental roles of some of their elements. One understudied component, the perineurium, ensheaths nerve fascicles and is a component of the blood-nerve-barrier. In zebrafish, the motor nerve perineurium is composed of CNS-derived nkx2.2a(+) perineurial glia, which establish the motor exit point (MEP) during development. To determine if mouse perineurial cells also originate within the CNS and perform a similar function, we created a Nkx2.2:EGFP transgenic reporter line.
Results In conjunction with RNA expression analysis and antibody labeling, we observed Nkx2.2(+) cells along peripheral motor nerves at all stages of development and in adult tissue. Additionally, in mice lacking Nkx2.2, we demonstrate that Nkx2.2(+) perineurial glia are essential for motor nerve development and Schwann cell differentiation.
Conclusions Our studies reveal that a subset of mouse perineurial cells are CNS-derived, express Nkx2.2, and are essential for motor nerve development. This work highlights an under-appreciated but essential contribution of CNS-derived cells to the development of the mammalian peripheral nervous system (PNS).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping