ZFIN ID: ZDB-PUB-140630-2
Light sheet microscopy
Weber, M., Mickoleit, M., Huisken, J.
Date: 2014
Source: Methods in cell biology   123C: 193-215 (Chapter)
Registered Authors: Huisken, Jan, Mickoleit, Michaela
Keywords: Developmental biology, Fluorescence microscopy, Image processing, Light sheet microscopy, Mounting, Selective plane illumination microscopy, Three-dimensional imaging, Ultramicroscopy, Zebrafish
MeSH Terms:
  • Animals
  • Humans
  • Image Enhancement
  • Imaging, Three-Dimensional/methods*
  • Microscopy, Confocal/methods
  • Microscopy, Fluorescence/methods
  • Time-Lapse Imaging/methods
PubMed: 24974029 Full text @ Meth. Cell. Biol.
This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample rotation. Based on our experience over the last 10 years, we summarize the key concepts in light sheet microscopy, typical implementations, and successful applications. In particular, sample mounting for long time-lapse imaging and the resulting challenges in data processing are discussed in detail.