PUBLICATION

Hooking the big one: the potential of zebrafish xenotransplantation to reform cancer drug screening in the genomic era

Authors
Veinotte, C.J., Dellaire, G., Berman, J.N.
ID
ZDB-PUB-140629-12
Date
2014
Source
Disease models & mechanisms   7: 745-754 (Review)
Registered Authors
Berman, Jason, Veinotte, Chansey
Keywords
Cancer, Drug screening, Microenvironment, Xenotransplantation, Zebrafish
MeSH Terms
  • Animals
  • Drug Screening Assays, Antitumor*
  • Genomics*
  • Humans
  • Neoplasms/drug therapy*
  • Neoplasms/genetics
  • Neoplasms/pathology
  • Transplantation, Heterologous*
  • Tumor Microenvironment
  • Zebrafish/genetics*
PubMed
24973744 Full text @ Dis. Model. Mech.
Abstract
The current preclinical pipeline for drug discovery can be cumbersome and costly, which limits the number of compounds that can effectively be transitioned to use as therapies. Chemical screens in zebrafish have uncovered new uses for existing drugs and identified promising new compounds from large libraries. Xenotransplantation of human cancer cells into zebrafish embryos builds on this work and enables direct evaluation of patient-derived tumor specimens in vivo in a rapid and cost-effective manner. The short time frame needed for xenotransplantation studies means that the zebrafish can serve as an early preclinical drug screening tool and can also help personalize cancer therapy by providing real-time data on the response of the human cells to treatment. In this Review, we summarize the use of zebrafish embryos in drug screening and highlight the potential for xenotransplantation approaches to be adopted as a preclinical tool to identify and prioritize therapies for further clinical evaluation. We also discuss some of the limitations of using zebrafish xenografts and the benefits of using them in concert with murine xenografts in drug optimization.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes