ZFIN ID: ZDB-PUB-140622-3
Mass Spectrometry for Proteomics-Based Investigation Using the Zebrafish Vertebrate Model System
Baral, R., Wetie, A.G., Darie, C.C., Wallace, K.N.
Date: 2014
Source: Advances in experimental medicine and biology   806: 331-340 (Chapter)
Registered Authors: Wallace, Kenneth
Keywords: none
MeSH Terms:
  • Animals
  • Genome/physiology*
  • Humans
  • Intestines/metabolism*
  • Mass Spectrometry/methods*
  • Mutation
  • Proteomics/methods*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 24952190 Full text @ Adv. Exp. Med. Biol.
The zebrafish (Danio rerio) is frequently being used to investigate the genetics of human diseases as well as resulting pathologies. Ease of both forward and reverse genetic manipulation along with conservation of vertebrate organ systems and disease causing genes has made this system a popular model. Many techniques have been developed to manipulate the genome of zebrafish producing mutants in a vast array of genes. While genetic manipulation of zebrafish has progressed, proteomics have been under-utilized. This review highlights studies that have already been performed using proteomic techniques and as well as our initial proteomic work comparing changes to the proteome between the ascl1a-/- and WT intestine.