PUBLICATION
Developmental hematopoiesis: ontogeny, genetic programming and conservation
- Authors
- Ciau-Uitz, A., Monteiro, R., Kirmizitas, A., Patient, R.
- ID
- ZDB-PUB-140621-2
- Date
- 2014
- Source
- Experimental hematology 42(8): 669-683 (Review)
- Registered Authors
- Patient, Roger K.
- Keywords
- Development, Hemangioblast, Hematopoietic stem cell, Hemogenic endothelium, Xenopus, Zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation
- Endothelial Cells/cytology
- Hematopoiesis*/genetics
- Hematopoietic Stem Cells/cytology
- Hematopoietic Stem Cells/physiology
- Humans
- Xenopus/embryology
- Zebrafish/embryology
- PubMed
- 24950425 Full text @ Exp. Hematol.
Citation
Ciau-Uitz, A., Monteiro, R., Kirmizitas, A., Patient, R. (2014) Developmental hematopoiesis: ontogeny, genetic programming and conservation. Experimental hematology. 42(8):669-683.
Abstract
Hematopoietic stem cells (HSCs) sustain blood production through life and are of pivotal importance in regenerative medicine. Although HSC generation from pluripotent stem cells would resolve their shortage for clinical applications, this has not yet been achieved mainly due to the poor mechanistic understanding of their programing. Bone marrow HSCs are first created during embryogenesis in the dorsal aorta (DA) of the mid-gestation conceptus, from where they migrate to the fetal liver and, eventually, the bone marrow. It is currently accepted that HSCs emerge from specialised endothelium, the hemogenic endothelium, localised in the ventral wall of the DA through an evolutionarily conserved process called the endothelial to hematopoietic transition (EHT). However, EHT represents one of the last steps in HSC creation and an understanding of earlier events in the specification of their progenitors is required if we are to create them from naïve pluripotent cells. Due to their ready availability and external development, studies on zebrafish and Xenopus embryos have enormously facilitated our understanding of the early developmental processes leading to the programming of HSCs from nascent lateral plate mesoderm to hemogenic endothelium in the DA. The amenity of the Xenopus model to lineage tracing experiments has also contributed to the establishment of the distinct origins of embryonic (yolk sac) and adult (HSC) hematopoiesis, whilst the transparency of the zebrafish has allowed in vivo imaging of developing blood cells, particularly during and after the emergence of HSCs in the DA. Here, we discuss the key contributions of these model organisms to our understanding of developmental hematopoiesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping