PUBLICATION
An Emerging Water Contaminant, Semicarbazide, Exerts an Anti-estrogenic Effect in Zebrafish (Danio rerio)
- Authors
- Gao, S., Wang, W., Tian, H., Zhang, X., Guo, L., Ru, S.
- ID
- ZDB-PUB-140616-1
- Date
- 2014
- Source
- Bulletin of environmental contamination and toxicology 93(3): 280-8 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Zebrafish/metabolism
- Female
- Receptors, Estrogen/metabolism*
- Semicarbazides/toxicity*
- Endocrine Disruptors/metabolism
- Endocrine Disruptors/toxicity*
- Animals
- Estrogens/metabolism
- Male
- Liver/metabolism
- Estradiol/metabolism
- Vitellogenins/metabolism*
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- PubMed
- 24929547 Full text @ Bull. Environ. Contam. Toxicol.
Citation
Gao, S., Wang, W., Tian, H., Zhang, X., Guo, L., Ru, S. (2014) An Emerging Water Contaminant, Semicarbazide, Exerts an Anti-estrogenic Effect in Zebrafish (Danio rerio). Bulletin of environmental contamination and toxicology. 93(3):280-8.
Abstract
To determine the endocrine disrupting effect of semicarbazide, an emerging water contaminant, the changes in transcript levels of hepatic estrogen-response genes including vitellogenin-1 (vtg-1), estrogen receptor α (ERα), and estrogen receptor β (ERβ) were measured in male and female zebrafish exposed to semicarbazide with or without exogenous 17β-estradiol (E2). Exposure of male zebrafish to semicarbazide for 96 h or 28 days resulted in no significant induction in hepatic vtg-1, ERα, or ERβ mRNA expression, indicating that semicarbazide has no estrogenic effect. However, a remarkable anti-estrogenic effect of semicarbazide was demonstrated: semicarbazide treatment of female zebrafish for 96 h and 28 days resulted in significant decreases in transcript levels of vtg-1, ERα, and ERβ, as well as decreases in the gonadosomatic index level after 28 days. Moreover, semicarbazide exposure significantly inhibited the induction of vtg-1, ERα and ERβ mRNA by E2 when male zebrafish were co-exposed for 28 days.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping