PUBLICATION

sdf1 expression reveals a source of perivascular-derived mesenchymal stem cells in zebrafish

Authors
Lund, T.C., Patrinostro, X., Kramer, A.C., Stadem, P., Higgins, L., Markowski, T.W., Wroblewski, M.S., Lidke, D.S., Tolar, J., Blazar, B.R.
ID
ZDB-PUB-140607-1
Date
2014
Source
Stem cells (Dayton, Ohio)   32(10): 2767-79 (Journal)
Registered Authors
Lund, Troy
Keywords
none
MeSH Terms
  • Animals
  • Biomarkers/metabolism
  • Blood Vessels/cytology*
  • Cell Differentiation
  • Cell Lineage
  • Cells, Cultured
  • Chemokine CXCL12/metabolism*
  • Human Umbilical Vein Endothelial Cells/cytology
  • Human Umbilical Vein Endothelial Cells/metabolism
  • Humans
  • Mesenchymal Stem Cells/cytology*
  • Mesenchymal Stem Cells/metabolism*
  • Mesoderm/cytology
  • Proteomics
  • Regeneration
  • Transgenes
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed
24905975 Full text @ Stem Cells
Abstract
There is accumulating evidence that mesenchymal stem cells (MSC) have their origin as perivascular cells (PVC) in vivo, but precisely identifying them has been a challenge, as they have no single definitive marker and are rare. We have developed a fluorescent transgenic vertebrate model in which PVC can be visualized in vivo based upon sdf1 expression in the zebrafish. Prospective isolation and culture of sdf1(DsRed) PVC demonstrated properties consistent with MSC including prototypical cell surface marker expression; mesodermal differentiation into adipogenic, osteogenic and chondrogenic lineages; and the ability to support hematopoietic cells. Global proteomic studies performed by 2-dimensional liquid chromatography and tandem mass spectrometry revealed a high degree of similarity to human MSC and discovery of novel markers (CD99, CD151 and MYOF) that were previously unknown to be expressed by hMSC. Dynamic in vivo imaging during fin regeneration showed that PVC may arise from undifferentiated mesenchyme providing evidence of a PVC - MSC relationship. This is the first model, established in zebrafish, in which MSC can be visualized in vivo and will allow us to better understand their function in a native environment. Stem Cells 2014.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping