PUBLICATION
Zebrafish-based systems pharmacology of cancer metastasis
- Authors
- Shimada, Y., Nishimura, Y., Tanaka, T.
- ID
- ZDB-PUB-140520-3
- Date
- 2014
- Source
- Methods in molecular biology (Clifton, N.J.) 1165: 223-38 (Chapter)
- Registered Authors
- Tanaka, Toshio
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Cell Transformation, Neoplastic
- Computational Biology
- Drug Screening Assays, Antitumor/methods*
- Fluorescent Dyes/metabolism
- Gene Expression Profiling
- Humans
- Molecular Imaging
- Neoplasm Metastasis
- Pancreatic Neoplasms/pathology
- Zebrafish*
- PubMed
- 24839028 Full text @ Meth. Mol. Biol.
Citation
Shimada, Y., Nishimura, Y., Tanaka, T. (2014) Zebrafish-based systems pharmacology of cancer metastasis. Methods in molecular biology (Clifton, N.J.). 1165:223-38.
Abstract
Because of their small size, high fecundity, and commonality to human genetics and genomics, phenotype-based animal testing using zebrafish (Danio rerio) has emerged as a powerful tool for identifying disease mechanisms, drug target molecules and small bioactive compounds over the last decade. Importantly, the immaturity of the zebrafish larvae immune system compared with that of mammals facilitates the implantation of human tumors representing aggressive cancer progression with metastasis. In the current chapter, we describe the methods for human cancer cell xenotransplantation into zebrafish, phenotypic image analysis, and transcriptome analysis using deep sequencing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping