|ZFIN ID: ZDB-PUB-140513-69|
Organizer-derived Bmp2 is required for the formation of a correct Bmp activity gradient during embryonic development
Xue, Y., Zheng, X., Huang, L., Xu, P., Ma, Y., Min, Z., Tao, Q., Tao, Y., Meng, A.
|Source:||Nature communications 5: 3766 (Journal)|
|Registered Authors:||Meng, Anming, Xu, Peng-Fei|
|PubMed:||24777107 Full text @ Nat. Commun.|
Xue, Y., Zheng, X., Huang, L., Xu, P., Ma, Y., Min, Z., Tao, Q., Tao, Y., Meng, A. (2014) Organizer-derived Bmp2 is required for the formation of a correct Bmp activity gradient during embryonic development. Nature communications. 5:3766.
ABSTRACTBone morphogenetic proteins (Bmps) control dorsoventral patterning of vertebrate embryos through the establishment of a ventrodorsal gradient of the activated downstream cytoplasmic effectors Smad1/5/8. Some Bmp ligands are expressed in the ventral and lateral regions and in the organizer during gastrulation of the embryo, but it remains unclear how organizer-derived Bmps contribute to total Bmp ligand levels and to the establishment of the correct phospho-Smad1/5/8 gradient along the ventrodorsal axis. Here we demonstrate that interference with organizer-specific Bmp2b signalling in zebrafish embryos alters the phospho-Smad1/5/8 gradient throughout the ventrodorsal axis, elevates the levels of the Bmp antagonist Chordin and dorsalizes the embryos. Moreover, we show that organizer-derived Bmp2b represses chordin transcription in the organizer and contributes to the control of the Chordin gradient. Combining these experimental results with simulations of Bmp's reaction-diffusion dynamics, our data indicate that organizer-produced Bmp2b is required for the establishment and maintenance of a Bmp activity gradient and for appropriate embryonic dorsoventral patterning during gastrulation.