PUBLICATION
Nrz but not zBcl-xL antagonizes Bcl-wav pro-apoptotic activity in zebrafish
- Authors
- Prudent, J., Gillet, G., Popgeorgiev, N.
- ID
- ZDB-PUB-140513-64
- Date
- 2014
- Source
- Communicative & integrative biology 7: e28008 (Journal)
- Registered Authors
- Gillet, Germain
- Keywords
- Apoptosis, Bcl-2 proteins, Bcl-wav, Nrz, zBcl-xL, zebrafish
- MeSH Terms
- none
- PubMed
- 24778760 Full text @ Commun. Integr. Biol.
Citation
Prudent, J., Gillet, G., Popgeorgiev, N. (2014) Nrz but not zBcl-xL antagonizes Bcl-wav pro-apoptotic activity in zebrafish. Communicative & integrative biology. 7:e28008.
Abstract
We recently identified a new highly divergent Bcl-2 related protein, named Bcl-wav, with phylogenetic pattern restricted to aquatic anamniotes. In zebrafish gastrula, bclwav gene silencing resulted in calcium-dependent cytoskeleton remodeling leading to convergence and extension movements defaults and abnormal orientation of the larva notochord. Beyond its function in cell migration, Bcl-wav was found to act as a pro-apoptotic Bcl-2 member inducing Bax/Bak dependent cell death. Here we show that, in zebrafish, pro-apoptotic Bcl-wav activity is selectively counteracted by the anti-apoptotic Nrz protein but not by zBcl-xL. Indeed Nrz but not zBcl-xL was able to decrease Bcl-wav dependent embryo mortality. Furthermore Nrz was able to prevent apoptosis induced by Bcl-wav ectopic expression in the embryo's head and tail. Finally co-immunoprecipitation experiments in HeLa cells showed that Bcl-wav directly interacts with Nrz. Overall these results expand our current knowledge about Bcl-2 family proteins interactome during early zebrafish development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping