PUBLICATION
Baicalein inhibits lipid accumulation by regulating early adipogenesis and m-TOR signaling
- Authors
- Seo, M.J., Choi, H.S., Jeon, H.J., Woo, M.S., Lee, B.Y.
- ID
- ZDB-PUB-140513-489
- Date
- 2014
- Source
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 67: 57-64 (Journal)
- Registered Authors
- Keywords
- 3T3-L1, Adipogenesis, Mitotic clonal expansion, Triglyceride signaling pathway, Zebrafish, m-TOR signaling pathway
- MeSH Terms
-
- TOR Serine-Threonine Kinases/metabolism*
- Adipogenesis*
- Signal Transduction*
- Lipid Metabolism*
- DNA Primers
- Base Sequence
- Flavanones/pharmacology*
- PubMed
- 24560969 Full text @ Food Chem. Toxicol.
- CTD
- 24560969
Citation
Seo, M.J., Choi, H.S., Jeon, H.J., Woo, M.S., Lee, B.Y. (2014) Baicalein inhibits lipid accumulation by regulating early adipogenesis and m-TOR signaling. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 67:57-64.
Abstract
Baicalein is a type of flavonoid that originates from Scutellaria baicalensis. In this study, we examined how baicalein inhibits lipid accumulation during adipogenesis in 3T3-L1 cells. Our data show that baicalein inhibited lipid accumulation during adipogenesis in a dose-dependent manner. Baicalein inhibition was limited to the early adipogenic stage. Cell cycle analysis showed that baicalein induced cell cycle arrest in the G0/G1 phase through cyclin downregulation. In addition, baicalein suppressed the mRNA expression of early adipogenic factors leading to downregulation of late adipogenic factors at mRNA and protein levels. Inhibition of adipogenic factors by baicalein was correlated with downregulation of lipid synthetic enzymes. Additionally, baicalein negatively regulated the m-TOR signaling pathway involved in lipid accumulation during adipogenesis, thus inhibiting phosphorylation of m-TOR and p70S6K. In a zebrafish study, baicalein significantly reduced lipid accumulation in Nile Red staining. Consistent with a report using cell lines, mRNA expression of adipogenic factors was decreased in a dose-dependent manner by baicalein. This result reflects a reduction in total triglyceride levels based on a triglyceride assay. Our data suggest that baicalein inhibits lipid accumulation by controlling the cell cycle and m-TOR signaling in 3T3-L1 cells, and its anti-adipogenic effect was found in a zebrafish model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping