PUBLICATION

Baicalein inhibits lipid accumulation by regulating early adipogenesis and m-TOR signaling

Authors
Seo, M.J., Choi, H.S., Jeon, H.J., Woo, M.S., Lee, B.Y.
ID
ZDB-PUB-140513-489
Date
2014
Source
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association   67: 57-64 (Journal)
Registered Authors
Keywords
3T3-L1, Adipogenesis, Mitotic clonal expansion, Triglyceride signaling pathway, Zebrafish, m-TOR signaling pathway
MeSH Terms
  • Adipogenesis*
  • DNA Primers
  • TOR Serine-Threonine Kinases/metabolism*
  • Base Sequence
  • Flavanones/pharmacology*
  • Lipid Metabolism*
  • Signal Transduction*
(all 7)
PubMed
24560969 Full text @ Food Chem. Toxicol.
CTD
24560969
Abstract
Baicalein is a type of flavonoid that originates from Scutellaria baicalensis. In this study, we examined how baicalein inhibits lipid accumulation during adipogenesis in 3T3-L1 cells. Our data show that baicalein inhibited lipid accumulation during adipogenesis in a dose-dependent manner. Baicalein inhibition was limited to the early adipogenic stage. Cell cycle analysis showed that baicalein induced cell cycle arrest in the G0/G1 phase through cyclin downregulation. In addition, baicalein suppressed the mRNA expression of early adipogenic factors leading to downregulation of late adipogenic factors at mRNA and protein levels. Inhibition of adipogenic factors by baicalein was correlated with downregulation of lipid synthetic enzymes. Additionally, baicalein negatively regulated the m-TOR signaling pathway involved in lipid accumulation during adipogenesis, thus inhibiting phosphorylation of m-TOR and p70S6K. In a zebrafish study, baicalein significantly reduced lipid accumulation in Nile Red staining. Consistent with a report using cell lines, mRNA expression of adipogenic factors was decreased in a dose-dependent manner by baicalein. This result reflects a reduction in total triglyceride levels based on a triglyceride assay. Our data suggest that baicalein inhibits lipid accumulation by controlling the cell cycle and m-TOR signaling in 3T3-L1 cells, and its anti-adipogenic effect was found in a zebrafish model.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Seo, M.J., Choi, H.S., Jeon, H.J., Woo, M.S., Lee, B.Y. (2014) Baicalein inhibits lipid accumulation by regulating early adipogenesis and m-TOR signaling. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 67:57-64.