PUBLICATION
Molecular effects of the cyanobacterial toxin cyanopeptolin (CP1020) occurring in algal blooms: global transcriptome analysis in zebrafish embryos
- Authors
- Faltermann, S., Zucchi, S., Kohler, E., Blom, J.F., Pernthaler, J., Fent, K.
- ID
- ZDB-PUB-140513-483
- Date
- 2014
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 149: 33-9 (Journal)
- Registered Authors
- Keywords
- Cyanobacterial toxin, Cyanopeptolin, Modes of action, Molecular effects, Transcriptomics, Zebrafish
- Datasets
- GEO:GSE50139
- MeSH Terms
-
- Animals
- Cluster Analysis
- Embryo, Nonmammalian
- Eutrophication*
- Gene Expression Profiling
- Peptides, Cyclic/analysis
- Peptides, Cyclic/toxicity*
- Reproducibility of Results
- Transcriptome/drug effects*
- Water Pollutants, Chemical/analysis
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 24561424 Full text @ Aquat. Toxicol.
Citation
Faltermann, S., Zucchi, S., Kohler, E., Blom, J.F., Pernthaler, J., Fent, K. (2014) Molecular effects of the cyanobacterial toxin cyanopeptolin (CP1020) occurring in algal blooms: global transcriptome analysis in zebrafish embryos. Aquatic toxicology (Amsterdam, Netherlands). 149:33-9.
Abstract
Higher water temperatures due to climate change combined with eutrophication of inland waters promote cyanobacterial blooms. Some of the cyanobacteria produce toxins leading to drinking water contamination and fish poisoning on a global scale. Here, we focused on the molecular effects of the cyanobacterial oligopeptide cyanopeptolin CP1020, produced by Microcystis and Planktothrix strains, by means of whole-genome transcriptomics. Exposure of 72 hpf old zebrafish embryos for 96 h to 100 and 1,000 μg/L CP1020 resulted in differential transcriptional alteration of 396 and 490 transcripts (fold change d 2), respectively, of which 68 gene transcripts were common. These belong to genes related to various important biological and physiological pathways. Most clearly affected were pathways related to DNA damage recognition and repair, circadian rhythm and response to light. Validation by RT-qPCR showed dose-dependent transcriptional alterations of genes belonging to DNA damage and repair and regulation of circadian rhythm. This leads to the hypothesis that CP1020 acts on DNA and has neurotoxic activity. This transcriptome analysis leads to the identification of novel and unknown molecular effects of this cyanobacterial toxin, including neurotoxicity, which may have important consequences for humans consuming contaminated drinking water.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping